Temperature dependence of potassium currents in isolated inner hair cells from guinea-pig cochlea

豚鼠耳蜗分离内毛细胞钾电流的温度依赖性

• 批准号: 21592160
• 负责人: KIMITSUKI Takashi
• 金额: $ 2.91万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21592160/
• 关键词: 内耳; 蝸牛; 内耳有毛細胞; カリウム電流; 体温; 活性化; 不活性; 有毛細胞; イオンチャネル; カリウムチャネル; 温度依存性; カルウムチャネル

The temperature dependence of potassium currents in isolated inner hair cells(IHCs) from mature guinea-pig cochlea was investigated. IHCs showed outwardly rectifying currents in response to depolarizing voltage pulses, with only a slight inward current when hyperpolarized. The amplitude of both outward and inward currents demonstrated no temperature dependency, however, activation and inactivation rates were faster at 36 oC than at room temperature. Half-time for activation was shorter at 36 oC than at room temperature at membrane potentials of-10,+ 10,+ 20,+ 30, and+ 40 mV. Q10 for the activation rate was 1. 83. The inactivation time constant in outward TEA-sensitive potassium currents was much smaller at 36 oC than at room temperature between the membrane potentials of-20 and+ 60 mV. Q10 for the inactivation time constant was 3. 19.

研究了豚鼠耳蜗内毛细胞钾电流的温度依赖性。IHC表现出外向整流电流响应去极化电压脉冲，只有轻微的内向电流时超极化。外向电流和内向电流的幅度均无温度依赖性，但36 ℃时的激活和失活速率比室温时快。在膜电位为-10、+ 10、+ 20、+ 30和+ 40 mV时，36 oC下的半激活时间比室温下短。激活率的Q10为1。83.在-20 mV和+60 mV的膜电位之间，36 oC时外向TEA敏感性钾电流的失活时间常数比室温时小得多。灭活时间常数的Q10为3。19.

项目成果

モルモット蝸牛内有毛細胞カリウム電流の不活性化過程-温度依存性について-

豚鼠耳蜗毛细胞钾电流失活过程-温度依赖性-

• 发表时间: 2009
• 作者: Furuya A;Hinohira Y;Katsuno M;Suzaki H.;君付隆
• 通讯作者: 君付隆

語音明瞭度と純音聴力検査閾値の比較

语音清晰度与纯音听力阈值的比较

• 发表时间: 2010
• 作者: 今井貴夫、西池季隆;他;君付隆
• 通讯作者: 君付隆

聴覚の基礎研究-何が分かって何が分かっていないのか

听力基础研究——什么是已知的，什么是未知的？

• 发表时间: 2010
• 作者: Makihara S;Okano M;Fujiwara T;Kariya S;Noda Y;Higaki T;Nishizaki K;君付隆
• 通讯作者: 君付隆

Mice lacking ganglioside GM3 synthase exhibit complete hearing loss due to selective degeneration of the organ of Corti

缺乏神经节苷脂 GM3 合酶的小鼠由于柯蒂氏器的选择性变性而表现出完全听力丧失

• 发表时间: 2009
• 期刊: Proc. Natrl. Acad. Sci. U.S.A. (In press)
• 作者: Yosikawa M.;Go S.;et al.
• 通讯作者: et al.

Property of I K, n in inner hair cells isolated from guinea-pig cochlea

• DOI: 10.1016/j.heares.2010.01.002
• 发表时间: 2010-03
• 期刊: Hearing Research
• 影响因子: 2.8
• 作者: T. Kimitsuki;N. Komune;T. Noda;Kazutaka Takaiwa;Mitsuru Ohashi;S. Komune