Cancer stem cell and EGFR target therapy for head and neck cancer

癌症干细胞和EGFR靶向治疗头颈癌

• 批准号: 21592211
• 负责人: FUJII Masato
• 金额: $ 3.16万
• 依托单位: 独立行政法人国立病院機構東京医療センター(臨床研究センター) (2011)独立行政法人国立病院機構(東京医療センター臨床研究センター) (2009-2010)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21592211/
• 关键词: 頭頸部癌; 幹細胞; EGFR; 癌幹細胞

In the EGFR expression in the head and neck cancer cell stock and the examination concerning the correlation with the MT1-MMP appearance, when EGFR was added to HSC4, an increase in the MT1-MMP appearance was admitted. That is, the possibility of taking part was suggested in the permeation proliferation of the tumor through not only the cell proliferation but also MT-MMP as for EGFR and because the correlation with MT1-MMP that related to the cancer stem cell had been admitted, the correlation of EGFR and the cancer stem cell was suggested. When the clone formative ability as the cancer stem cell of HSC4 was examined, 26% cells had the clone formative ability and the clone of 60% was cause ALP on the inside positivity. Expression of Oct3/4 and Nanog as cancer stem cell marker in HSC4 was proved. The SP cell was identified from HSC4 by FACS. As a result, SP cell of 0.3-0.4% was identified. The appearance of Oct3/4 and Nanog was accentuation in the SP cell in significant as a result of e … More xamining the various cancer stem cell markers appearance in the SP cell and the non-SP cell that the separation identification is done with FACS. The SP cell and the MP cell were identified from head neck cancer cell SAS, SCC4 apart and the cancer stem cell marker s appearance was examined. The appearance of Oct3/4 and Nanog was up regulated as a result in significant as well as HSC4.Cancer stem cell marker appearance and the lymph node in 41 examples of stage I/II of the early stage of the example of the tongue cancer syndrome, the correlation with metastasis(DNM) was examined. As a result, it intentionally correlated to DNM, Oct3/4, and the Nanog appearance. After various and pathology features, the cancer stem cell marker appearance, and lymph nodes in the early stage tongue cancer, the correlation with metastasis(DNM) was examined by the multivariate analysis. As a result, the vessel invasion of the tumor and Oct3/4 were proven and it was proven to DNM that it was an independent risk factor. As for EGFR, it was suggested that Oct3/4 be deeply related to the recurrence of cancer and the metastasis as the cancer stem cell marker in relation to permeation and the metastasis of cancer through EGFR above. Less

在头颈部癌细胞库中的EGFR表达和关于与MT 1-MMP出现的相关性的检查中，当EGFR加入HSC 4时，MT 1-MMP出现增加。也就是说，关于EGFR，不仅通过细胞增殖而且通过MT-MMP参与肿瘤的渗透性增殖的可能性被提出，并且因为已经承认与癌干细胞相关的MT 1-MMP的相关性，所以EGFR和癌干细胞的相关性被提出。以HSC 4为癌干细胞检测克隆形成能力，26%的细胞具有克隆形成能力，60%的克隆内ALP阳性。证实了Oct 3/4和Nanog作为癌干细胞标志物在HSC 4中的表达。流式细胞仪鉴定SP细胞为HSC 4。结果，鉴定出0.3-0.4%的SP细胞。结果显示，在SP细胞中Oct 3/4和Nanog的出现明显增强， ...更多信息 用流式细胞仪检测SP细胞和非SP细胞中各种肿瘤干细胞标志物的出现。分别从头颈癌细胞SAS、SCC 4中鉴定出SP细胞和MP细胞，并检测肿瘤干细胞标志物的出现。Oct 3/4和Nanog的出现上调，结果与HSC 4一样显著。对41例舌癌综合征早期I/II期患者的癌干细胞标志物出现与淋巴结转移的相关性（DNM）进行了检测。因此，它故意与DNM，Oct 3/4和Nanog外观相关。通过多因素分析，对早期舌癌的各种病理特征、癌干细胞标志物的出现、淋巴结转移与转移（DNM）的相关性进行了研究。结果，证实了肿瘤的血管浸润和Oct 3/4，并向DNM证明了这是一个独立的危险因素。对于EGFR，提示Oct 3/4作为肿瘤干细胞标志物，与肿瘤的复发和转移密切相关，并通过上述EGFR与肿瘤的渗透和转移有关。少

项目成果

頭頸部扁平上皮癌細胞株における上皮成長因子受容体と膜型MMPの相互作用

头颈鳞癌细胞系表皮生长因子受体与膜型基质金属蛋白酶的相互作用

• 发表时间: 2009
• 作者: Nakazawa M;Ohguro H;Takeuchi K;4Miyagawa Y;Ito T;Metoki T;羽生昇
• 通讯作者: 羽生昇

頭頸部扁平上皮癌におけるがん幹細胞の存在とmakerの発現に関する検討

头颈鳞状细胞癌中肿瘤干细胞的存在及标记物表达的研究

• 发表时间: 2011
• 作者: Yoshitake;et al.;Yoshitake H. Maruyama M. Yanagida M. Takamori K. Ogawa H. Hasegawa A. Araki Y.;吉武洋・丸山真由子・柳田光昭・高森建二・長谷川昭子・荒木慶彦;丸山真由子・吉武洋・高森建二・小川秀興・荒木慶彦;羽生昇藤井正人今西順久徳丸裕小川郁;羽生昇今西順久徳丸裕坂本耕二冨田俊樹小川郁藤井正人
• 通讯作者: 羽生昇今西順久徳丸裕坂本耕二冨田俊樹小川郁藤井正人

頭頸部扁平上皮癌におけるSP細胞の同定と機能の解明

头颈鳞状细胞癌SP细胞的鉴定及功能阐明

• 发表时间: 2010
• 作者: Sato K;et al;羽生昇徳丸裕藤井正人
• 通讯作者: 羽生昇徳丸裕藤井正人

口腔舌扁平上皮癌におけるOct3/4とNanogの発現とその臨床的意義

Oct3/4和Nanog在口腔舌鳞状细胞癌中的表达及其临床意义

• 发表时间: 2011
• 作者: Yoshitake;et al.;Yoshitake H. Maruyama M. Yanagida M. Takamori K. Ogawa H. Hasegawa A. Araki Y.;吉武洋・丸山真由子・柳田光昭・高森建二・長谷川昭子・荒木慶彦;丸山真由子・吉武洋・高森建二・小川秀興・荒木慶彦;羽生昇藤井正人今西順久徳丸裕小川郁
• 通讯作者: 羽生昇藤井正人今西順久徳丸裕小川郁

頭頸部扁平上皮癌における癌幹細胞用細胞の存在とmarkerの発言に関する検討

头颈鳞状细胞癌中癌症干细胞的存在和标记物的检查

• 发表时间: 2011
• 作者: SATO Kiminori;UMENO Hirohito;NAKASHIMA Tadashi;NONAKA Satoshi;HARABUCHI Yasuaki;羽生昇
• 通讯作者: 羽生昇