Interaction between MT1-MMP and CD44 in the invasion and metastasis of head and neck cancer

MT1-MMP与CD44在头颈癌侵袭转移中的相互作用

• 批准号: 13671800
• 负责人: FUJII Masato
• 金额: $ 2.11万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671800/
• 关键词: Head and Neck Cancer; MTI-MMP; CD44

We investigated the expression of MT 1,2,3-MMPs in the head and neck cancer cell line in vitro. The expression of MT1,2,3-MMPs was also studied for the surgical specimen using Northern blotting, immuno-histochemical study, and in situ hybridization. The expression of CD44 v6 and CD44 v2 was studied by immuno-histochemical method. MT1-MMP mRNA was expressed in all of five head and neck cancer cell lines. The level of MT1-MMP mRNA expression was closely correlated to the rate of lymph node metastasis (p=0.0081). The relation between over expression of MT1-MMP mRNA and lymph node metastasis was statistically significant in multivariate analysis. The expression of CD44v6 was also correlated with lymph node metastasis, and the expression of CD44v2 was correlated with poor prognoses. The expression of MT1-MMP is related to metastasis of head and neck cancer, and the expressions of CD44 v6, v2 are closely related to the biological malignancy of head and neck cancer. The relation between the expression of MT1-MMP and CD44 is important to evaluate the malignant potential of head and neck cancer.

我们研究了MT 1，2，3-MMPs在体外培养的头颈癌细胞系中的表达。采用北方印迹法、免疫组化法和原位杂交法检测手术标本中MT 1，2，3-MMPs的表达。免疫组化法检测CD 44 v6和CD 44 v2的表达。MT 1-MMP mRNA在5种头颈癌细胞系中均有表达。MT 1-MMPmRNA表达水平与淋巴结转移率密切相关（p=0.0081）。多因素分析显示MT 1-MMPmRNA过表达与淋巴结转移有显著相关性。CD 44 v6的表达与淋巴结转移有关，CD 44 v2的表达与淋巴结转移不良有关。MT 1-MMP的表达与头颈部肿瘤的转移有关，CD 44 v6、v2的表达与头颈部肿瘤的生物学恶性程度密切相关。MT 1-MMP与CD 44表达的关系对于评价头颈部癌的恶性潜能具有重要意义。

项目成果

Masato Fujii: "Significance of Epidermal growth factor receptor and tumor associated tissue eosinophilia in the prognosis of NPC"Auris Nasus Larynx. 29. 175-181 (2002)

Masato Fujii：“表皮生长因子受体和肿瘤相关组织嗜酸性粒细胞增多在鼻咽癌预后中的意义”Auris Nasus Larynx。

山下拓, 藤井正人 他: "上顎洞扁平上皮癌の臨床統計学的検討"日本耳鼻咽喉科学会会報. 105. 732-740 (2002)

Taku Yamashita、Masato Fujii 等：“上颌窦鳞状细胞癌的临床和统计研究”日本耳鼻喉科学会通报 105. 732-740 (2002)。

藤井 正人 他: "頭頸部扁平上皮癌におけるCD44変異体発現とその役割"日本耳鼻咽喉科学会会報. 104巻4号. 416-416 (2001)

Masato Fujii 等：“CD44 变异表达及其在头颈鳞状细胞癌中的作用”日本耳鼻喉科协会通报，第 104 卷，第 4 期，416-416（2001 年）。

Masato Fujii et al.: "Significance of epidermal growth factor receptor and tumor associated tissue eosinophilia in the prognosis of patients with nasopharyugeal carcinoma"Auris Nasus Larynx. 29・2. 175-181 (2002)

Masato Fujii 等：“表皮生长因子受体和肿瘤相关组织嗜酸性粒细胞增多对鼻咽癌患者预后的意义”Auris Nasus Larynx. 175-181 (2002)。

Masato Fujii et al.: "Significance of epidermal growth factor receptor and tumor associated tissue eosinophilia in the prognosis of NPC"Auris Nasus Larynx. 29.2. 175-181 (2002)

Masato Fujii 等人：“表皮生长因子受体和肿瘤相关组织嗜酸性粒细胞增多在鼻咽癌预后中的意义”Auris Nasus Larynx。