Analysis of regulatory mechanism of bone destruction and inflammation in experimental periodontitis mediated by IL-27 receptor

IL-27受体介导的实验性牙周炎骨破坏及炎症调控机制分析

• 批准号: 21592366
• 负责人: KUKITA Akiko
• 金额: $ 2.83万
• 依托单位: Saga University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21592366/
• 关键词: 破骨細胞; 歯周病; 細菌感染; 骨破壊; サイトカイン; 歯周炎; ノックアウトマウス; マクロファージ; IL-27; T細胞; 病的骨破壊; 免疫

WSX-1 gene is a subunit of receptor for IL-27 which is involved in the regulation of immunity and inflammation. We analyzed the role of IL-27 in bone destruction of periodontitis using WSX-1 knocked-out mice. Mice were infected with Porphyromonas gingivalis which is one of major pathogens in periodontitis. Alveolar bone loss following P. gingivalis was enhanced in WSX-1 KO mice compared to those in wild-type mice. CD4-positive T cells in spleen of periodontitis mice produced higher amount of IL-17, and the production of IFN-γand IL-17 was further promoted in WSX-1 KO mice. P. gingivalis infection promoted differentiation from macrophages into osteoclasts, and osteoclast formation induced by infection was enhanced in macrophage of WSX-1 KO mice. The data suggest that signals through IL-27 receptor are involved in bone loss of periodontitis by affecting osteoclastogenesis directly and indirectly.

WSX-1基因是IL-27受体的一个亚单位，参与免疫和炎症的调节。我们使用WSX-1基因敲除小鼠分析了IL-27在牙周炎骨破坏中的作用。牙龈卟啉单胞菌是引起牙周炎的主要病原菌之一。与野生型小鼠相比，WSX-1 KO小鼠中牙龈卟啉单胞菌引起的牙槽骨丢失增加。牙周炎小鼠脾脏中CD 4 + T细胞产生IL-17的量增加，WSX-1 KO小鼠IFN-γ和IL-17的产生进一步增加。牙龈卟啉单胞菌感染促进巨噬细胞向破骨细胞分化，并且感染诱导的破骨细胞形成在WSX-1 KO小鼠的巨噬细胞中增强。提示IL-27受体信号通过直接或间接影响破骨细胞的生成，参与牙周炎的骨丢失。

项目成果

破骨細胞特異的モノクローナル抗体Kat1を用いた破骨細胞前駆細胞の性状解析

使用破骨细胞特异性单克隆抗体 Kat1 表征破骨细胞前体细胞

• 发表时间: 2010
• 作者: 根本孝幸;小野俊雄;下山佑;木村重信;根本優子;松原麗et al
• 通讯作者: 松原麗et al

ホスファチジルセリンリポソームによる骨形成の促進

磷脂酰丝氨酸脂质体促进骨形成

• 发表时间: 2010
• 作者: 馬紅梅;武洲;李銀姫;久木田敏夫;中西博
• 通讯作者: 中西博

ヒト間葉系幹細胞による炎症性破骨細胞分化の制御とビスホスホネートの影響

人间充质干细胞对炎症破骨细胞分化的调节及双磷酸盐的影响

• 发表时间: 2010
• 作者: 斧美緒;他
• 通讯作者: 他

A possible suppressive role of galectin-3 in upregulated osteoclastogenesis accompanying adjuvant-induced arthritis in rats

• DOI: 10.1038/labinvest.2008.111
• 发表时间: 2009-01-01
• 期刊: LABORATORY INVESTIGATION
• 影响因子: 5
• 作者: Li, Yin-Ji;Kukita, Akiko;Kukita, Toshio
• 通讯作者: Kukita, Toshio

Phosphatidylserine-Containing Liposomes Inhibit the Differentiation of Osteoclasts and Trabecular Bone Loss

• DOI: 10.4049/jimmunol.0803609
• 发表时间: 2010-03
• 期刊: The Journal of Immunology
• 作者: Zhou Wu;Hong-Mei Ma;T. Kukita;Y. Nakanishi;H. Nakanishi