Study of SP cells under hypoxic conditions and molecular targeted therapy

低氧条件下SP细胞及分子靶向治疗的研究

• 批准号: 21592558
• 负责人: KAWASAKI Goro
• 金额: $ 2.83万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21592558/
• 关键词: SP細胞; 低酸素環境; 扁平上皮癌細胞; 口腔癌; mTOR; HIF1α; VEGF; TS; KAI1; 分子標的; 低酸素; 分子標的治療

Side population (SP) cells were isolated from oral cancer cell lines. SP cells possessed high malignant ability and demonstrated more drug resistance to 5-FU, as compared with non-SP cells. Under hypoxic conditions, HIF-1 expressions was associated with pathological malignancy grades, particularly activation of mTOR-HIF-1α-VEGF pathway was associated with pathological malignancy grades. Oral squamous cell carcinoma cell lines were treated with mTOR inhibitor, thereafter carcinoma cells revealed cell growth inhibition and reduced invasion. Under hypoxic conditions, HIF-1α was activated. The expression of mTOR-HIF-1α-VEGF pathway under hypoxic conditions was decreased by the treatment with mTOR inhibitor.

从口腔癌细胞系中分离侧群（SP）细胞。与非SP细胞相比，SP细胞具有更高的恶性能力，对5-FU的耐药性更强。缺氧条件下，HIF-1表达与病理恶性程度相关，尤其是mTOR-HIF-1α-VEGF通路的激活与病理恶性程度相关。用mTOR抑制剂处理口腔鳞状细胞癌细胞系，此后癌细胞显示细胞生长抑制和侵袭减少。低氧条件下，HIF-1α被激活。mTOR抑制剂可降低缺氧条件下mTOR-HIF-1α-VEGF通路的表达。

项目成果

mTOR阻害薬 everolimusの口腔扁平上皮癌細胞株に対する抗腫瘍効果について

mTOR抑制剂依维莫司对口腔鳞癌细胞系的抗肿瘤作用

• 发表时间: 2012
• 作者: 宮下仁;森士朗;李麗;堀江佐知子;渡邉夕紀子;大木宏介;川村仁;森川秀広;小玉哲也;鳴瀬智史,川崎五郎,柳本惣市,山田慎一,吉冨 泉,六反田賢,川北晃子,梅田正博
• 通讯作者: 鳴瀬智史,川崎五郎,柳本惣市,山田慎一,吉冨 泉,六反田賢,川北晃子,梅田正博

Isolation and characterization of cancer stem-like side population cells in human oral cancer cells

• DOI: 10.1016/j.oraloncology.2011.06.501
• 发表时间: 2011-09-01
• 期刊: ORAL ONCOLOGY
• 影响因子: 4.8
• 作者: Yanamoto, Souichi;Kawasaki, Goro;Umeda, Masahiro
• 通讯作者: Umeda, Masahiro

Immunohistochemical study of VEGF expression in oral squamous cell carcinomas: correlation with the mTOR-HIF-1α pathway.

• 发表时间: 2011-12
• 期刊: Anticancer research
• 影响因子: 2
• 作者: T. Naruse;G. Kawasaki;S. Yanamoto;A. Mizuno;M. Umeda

口腔扁平上皮癌におけるVEGF-AおよびVEGF-Cの発現に関する免疫組織化学的研究 : mTOR-HIF-1α経路との関連について

口腔鳞状细胞癌中VEGF-A和VEGF-C表达的免疫组化研究：与mTOR-HIF-1α通路的关系

• 发表时间: 2010
• 作者: 川崎五郎、鳴瀬智史;他
• 通讯作者: 他

Expression of Thymidylate Synthase and Dehydropyrimidine Dehydrogenase in Primary Oral Squamous Cell Carcinoma and Corresponding Metastases in Cervical Lymph Nodes: Association with the Metastasis Suppressor CD82

胸苷酸合酶和脱氢嘧啶脱氢酶在原发性口腔鳞状细胞癌和颈部淋巴结相应转移灶中的表达：与转移抑制因子 CD82 的关联

• 发表时间: 2011
• 期刊: Anticancer Res
• 影响因子: 2
• 作者: Kawasaki;G.;Yoshitomi;I. Yanamoto;S.;Yamada;S.;Mizuno;A;Umeda;M
• 通讯作者: M