Development of new oral cancer treatment as molecular target for antiapoptosis protein and NF-kB

XIAP是凋亡抑制蛋白家族的一员，通过阻断caspase介导的细胞凋亡与细胞存活相关。XIAP在多种恶性肿瘤中均有表达。据报道，XIAP的过度表达在多种恶性肿瘤中是一个较差的预后因素。本研究旨在探讨XIAP蛋白在口腔鳞状细胞癌(OSCC)中的表达及其与临床分期、组织分化程度及侵袭方式分类的关系。本研究以人口腔鳞癌细胞系为研究对象。以正常牙龈上皮细胞为对照。免疫印迹法检测培养细胞XIAP、cIAPs和Survivin的表达。组织标本取自85例口腔鳞癌患者手术或活检后的标本。用免疫组织化学方法检测它们的表达。在所有癌细胞中都检测到了这些蛋白的表达，但在正常细胞中没有检测到。对85例口腔鳞癌进行免疫组织化学分析，73例(86%)表达XIAP。XIAP的表达与临床分期、侵袭方式分类无关。XIAP的表达与组织分化程度有显著差异。大多数无染色和弱染色的癌组织分化较好。相反，在低分化癌中常可见强而广泛的染色。

XIAP is a member of the inhibitor of apoptosis protein family, which is associated with cell survival by blocking caspase-mediated apoptosis. XIAP is expressed in various malignant tumors. The overexpression of XIAP has been reported to be a poorer prognostic factor in various malignancies. present study were to evaluate the expression of XIAP protein in oral squamous cell carcinoma(OSCC) and to elucidate the relationships among the XIAP expression, clinical stages, histological differentiation and classification of invasion mode. human OSCC cell lines were used in this study. Normal gingival epithelial cells served as control. XIAP, cIAPs and survivin expressions of cultured cells wert detected by western blot. Tissue specimens were obtains from 85 patients with OSCC after surgery or biopsy. Their expression was detected by an immunohistochemical method. The expression of those proteins was detected in all cancer cells, but not in normal cells. Immunohistochemical analysis of 85 cases of OSCC showed that 73(86%) cases expressed XIAP. There was no relationship between XIAP expression and clinical stages, or classification of invasion mode. There were significant differences between XIAP expression and histological differentiation. Most of non-staining and weakly staining of cancer was well differentiated. In contrast, intense and extensive staining was frequently found in poorly differentiated cancer.