Identification of mechanisms for bone metabolism regulated by enamel matrix proteins. a possibility of estrogen's involvement

鉴定牙釉质基质蛋白调节骨代谢的机制。

• 批准号: 21592588
• 负责人: IGARASHI Koaoru
• 金额: $ 2.91万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21592588/
• 关键词: アメロジェニン; エナメルタンパク; 骨代謝; エストロゲン; 歯学

Recent studies suggest that the amelogenins are expressed in various tissues including bone and affecting bone metabolism. In this study, we examined the effects of LRAP, one of the major splice isoform of amelogenins, on stimulated bone turnover under estrogen deficiency. Using mesenchymal cells isolated from wild type and LRAP transgenic mice with or without ovariectomy(OVX), gene expression levels of inter leukin-6(IL-6), cycrooxigenase-2(COX2), and nitric oxide (NO)synthase were examined by real-time PCR. In addition, ELISA or colorimetric assay was also performed to measure prostaglandin E_2(PGE_2)or NO production from the cells. We found that the LRAP inhibited the elevation of IL-6 and COX2 gene expression, which is typically found in the estrogen deficient condition. PGE_2 production was decreased in the ovariectomized LRAP TG derived cells, but not in the ovariectomized wild type derived cells. The production of NO was increased in the ovariectomized LRAP TG cells. The LRAP may play a significant role on the prevention from bone loss due to estrogen deficiency, by changing the interleukin and PGE_2 expression, and the production on NO

近年来的研究表明，釉原蛋白在包括骨在内的多种组织中表达，并影响骨代谢。在这项研究中，我们研究了LRAP，釉原蛋白的主要剪接异构体之一，在雌激素缺乏刺激骨转换的影响。使用分离自野生型和LRAP转基因小鼠（有或没有卵巢切除术（OVX））的间充质细胞，通过实时PCR检测白细胞介素-6（IL-6）、环氧化酶-2（COX 2）和一氧化氮（NO）合酶的基因表达水平。此外，还用ELISA或比色法测定细胞产生的前列腺素E_2（PGE_2）或NO。我们发现LRAP抑制IL-6和COX 2基因表达的升高，这通常在雌激素缺乏的情况下发现。去卵巢的LRAP TG衍生细胞PGE_2的产生减少，但不影响去卵巢的野生型衍生细胞。去卵巢LRAP TG细胞NO生成增加。LRAP可能通过改变IL、PGE_2的表达及NO的产生，对雌激素缺乏引起的骨丢失起到一定的预防作用

项目成果

A method for rapid demineralization of teeth and bones.

• DOI: 10.2174/1874210601004010223
• 发表时间: 2010-12-15
• 期刊: The open dentistry journal
• 作者: Cho A;Suzuki S;Hatakeyama J;Haruyama N;Kulkarni AB
• 通讯作者: Kulkarni AB

Accuracy of orthodontic miniscrew implantation guided by stereolithographic surgical stent based on cone-beam CT-derived 3D images

• DOI: 10.2319/033111-231.1
• 发表时间: 2012-03-01
• 期刊: ANGLE ORTHODONTIST
• 影响因子: 3.4
• 作者: Qiu, Ling Ling;Haruyama, Naoto;Moriyama, Keiji
• 通讯作者: Moriyama, Keiji

筋ジストロフィーマウスにおける筋機能活性化が下顎骨の成長に与える影響

肌肉功能激活对肌营养不良小鼠下颌生长的影响

• 发表时间: 2009
• 作者: 三戸天元;春山直人;五十嵐薫
• 通讯作者: 五十嵐薫

アメロジェニンスプライスアイソフォームの軟骨細胞分化に与える影響

牙釉蛋白剪接亚型对软骨细胞分化的影响

• 发表时间: 2010
• 作者: 三谷薫子;春山直人;五十嵐薫
• 通讯作者: 五十嵐薫

Chapter 3, Lessons from the amelogenin knockout mice. In : Amelogenins : multifaceted proteins for dental and bone formation and repair

第三章，釉原蛋白敲除小鼠的教训。

• 发表时间: 2010
• 作者: Haruyama N;Hatakeyama J;Hatakeyama Y;Gibson CW;Kulkarni AB.
• 通讯作者: Kulkarni AB.