Investigation of stretch-induced intracellular signal transduction in human periodontal ligament fibroblasts exposed to periodontopathic factors.

研究暴露于牙周病因子的人牙周膜成纤维细胞中拉伸诱导的细胞内信号转导。

• 批准号: 21592617
• 负责人: YAGI Takakazu
• 金额: $ 2.83万
• 依托单位: Kagoshima University (2010-2011)Asahi University (2009)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21592617/
• 关键词: メカニカルストレス; ヒト歯根膜細胞線維芽細胞; 細胞内シグナル伝達機構; 歯根膜細胞; 炎症性シグナリングカスケード; 歯根膜線維芽細胞; シグナリングカスケード

In this study, we investigated the mechanism of intracellular signaling cascade with mechanical stress using the human periodontal ligament fibroblast(HPLF). And we investigated the cellular response of the active center of lipopolysaccharide(LPS) endotoxin which is a virulence factor of periodontal disease. Moreover, we researched the effect of mechanical stress against these cell receptors. As a result, we confirmed that HPLFs were expressed on the inflammatory cytokines via the MAPK signaling cascade in the uni-axial stretch. Next, we found that the expression of inflammatory cytokines through the MAPK signaling cascade was enhanced by the mechanical stress after stimulating by the virulence factors of periodontal disease.Therefore, it is suggested that the cell receptor against the center of virulence of LPS might be affected by the mechanical deformation. Further study is required in the future.

本研究以人牙周膜成纤维细胞（HPLF）为研究对象，探讨机械应力对细胞内信号级联反应的影响机制。并探讨了牙周病毒力因子脂多糖（LPS）内毒素活性中心的细胞反应。此外，我们还研究了机械应力对这些细胞受体的影响。因此，我们证实了在单轴拉伸中，HPLFs通过MAPK信号级联在炎性细胞因子上表达。其次，我们发现牙周病毒力因子刺激后，机械应力可通过MAPK信号级联途径增强炎症细胞因子的表达，提示机械变形可能影响了LPS毒力中心的细胞受体。今后需要进一步研究。

项目成果

Bioactive mechanism of Porphyromonas gingivalis lipid A

• DOI: 10.1111/j.1600-0757.2009.00343.x
• 发表时间: 2010-01-01
• 期刊: PERIODONTOLOGY 2000
• 影响因子: 18.6
• 作者: Ogawa, Tomohiko;Yagi, Takakazu
• 通讯作者: Yagi, Takakazu

歯周病原性因子存在下における培養ヒト歯根膜線維芽細胞への物理的刺激による細胞内シグナル伝達系の動態検討Investigation of stretch-induced intracellular signal transduction in human periodontal ligament fibroblasts exposed to periodontopathic factors

暴露于牙周病因子的人牙周膜成纤维细胞中拉伸诱导的细胞内信号转导的研究

• 发表时间: 2011
• 作者: 八木孝和;前田綾;植田紘貴;菅真有;宮脇正一
• 通讯作者: 宮脇正一

歯周病原性因子存在下における培養ヒト歯根膜線維芽細胞への物理的刺激による細胞内シグナル伝達系の動態検討

牙周致病因子存在下物理刺激培养的人牙周膜成纤维细胞研究细胞内信号转导系统的动态

• 发表时间: 2011
• 作者: 八木孝和;前田綾;植田紘貴;菅真由;宮脇正一
• 通讯作者: 宮脇正一

Patient with oculo-fecio-cardio-dental syndrome treated with surgical orthodontics

接受手术正畸治疗的眼粪心齿综合征患者

• 发表时间: 2012
• 期刊: Am J Orthod Dentofacial Orthop
• 作者: Sakaguchi K;Takakazu Y;Nagata J;Kubota T;Sugihara K;Miyawaki S
• 通讯作者: Miyawaki S

Patient with oculo-facio-cardio-dental syndrome treated with surgical orthodontics

• DOI: 10.1016/j.ajodo.2011.10.020
• 发表时间: 2012-04-01
• 期刊: AMERICAN JOURNAL OF ORTHODONTICS AND DENTOFACIAL ORTHOPEDICS
• 影响因子: 3
• 作者: Sakaguchi, Katsuyoshi;Yagi, Takakazu;Miyawaki, Shouichi
• 通讯作者: Miyawaki, Shouichi