Molecular biological study on the mechanism of alveolar bone metabolism through the toll-like receptor 5

Toll样受体5影响牙槽骨代谢机制的分子生物学研究

• 批准号: 21592639
• 负责人: NAKAMURA Kimiya
• 金额: $ 2.58万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21592639/
• 关键词: 予防歯科学; 歯槽骨; Toll様受容体5; フラジェリン; MCP-1; OPG; 歯周疾患; 骨芽細胞

Flagellin, the ligand of Toll like receptor 5, markedly increased the expression of MCP-1 which is one of the CC chemokine, in a dose-dependent manner on osteoblastic MC3T3-E1 cells. In addition, flagellin was significantly suppressed the OPG mRNA expression. Furthermore, anti-MCP-1 antibody was further down-regulated the flagellin-suppressed OPG mRNA expression. These results indicate that osteoblasts are involved in host defense as well as bone metabolism.

Toll样受体5的配体鞭毛蛋白显著增加成骨细胞MC3T3-E1细胞中CC趋化因子MCP-1的表达，并呈剂量依赖关系。此外，鞭毛蛋白还能显著抑制OPG基因的表达。此外，抗MCP-1抗体进一步下调鞭毛蛋白抑制的OPG mRNA的表达。这些结果表明，成骨细胞参与宿主防御和骨代谢。

项目成果

骨芽細胞様MC3T3-E1細胞におけるTLR5リガンドによるMCP-1の発現誘導

TLR5 配体在成骨细胞样 MC3T3-E1 细胞中诱导 MCP-1 表达

• 发表时间: 2011
• 作者: Matsumura Y;Mitani A;Suga T;Kamiya Y;Kikuchi T;Tanaka S;Aino M;Noguchi T;中村公也,竹原順次,三宅亮,兼平孝
• 通讯作者: 中村公也,竹原順次,三宅亮,兼平孝

Flagellin induces MCP-1 in mouse osteoblastic cells

鞭毛蛋白在小鼠成骨细胞中诱导 MCP-1

• 发表时间: 2010
• 作者: Kimiya Nakamura;Yoshiaki Deyama;Yoshitaka Yoshimura;Kuniaki Suzuki
• 通讯作者: Kuniaki Suzuki

Toll like receptor 5 ligand induces monocyte chemoattractant protein-1 in mouse osteoblastic cells

Toll样受体5配体诱导小鼠成骨细胞中的单核细胞趋化蛋白-1

• DOI: 10.2220/biomedres.33.39
• 发表时间: 2012
• 期刊: Biomedical Research
• 作者: Nakamura K;Deyama Y;Yoshimura Y;Suzuki K;Morita M
• 通讯作者: Morita M