Comparative evolutionary studies on three-dimensionally positioning of the chromosome territories in the primate iPS cell nuclei

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In this study from the viewpoint of spatial positioning of chromosome territories and genomic regions within the nucleus I would try to find the molecular mechanism of why the iPS cells are established and to identify what the human specific characteristics have been appeared during the course of primate evolution by comparative analysis in human and great apes. Analysis by 3D-FISH technique using the DNA probes with Oct3/4, Sox2, Klf4, c-Myc, and Nanog, and their chromosomal paints onto human, chimpanzee, gorilla, and orangutan fibroblast cell nuclei, basic characteristics of spatial arrangements have been revealed but analysis has not yet been carried out due to shortage of primate iPS cells. When accumulating iPS cells, I would perform the analyses and discuss the issues.