权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Genomic architecture and evolutionary organization by three-dimensional nuclear positioning of chromosome territories in mammalian species especially in primates

哺乳动物，特别是灵长类动物中染色体区域的三维核定位的基因组结构和进化组织

基本信息

批准号：
16510143
负责人：
TANABE Hideyuki
金额：
$ 2.5万
依托单位：
The Graduate University for Advanced Studies (Sokendai)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16510143/
关键词：
chromosome nuclear architecture primates chromosome territory FISH genome evolution cell culture 3D-FISH 染色体テリトリ

项目摘要

Chromosome territories (CTs) in mammalian cell nuclei are highly compartmentalized as distinct domains and visualized by three-dimensional fluorescence in situ hybridization (3D-FISH) technique. The radial distribution of CTs is highly correlated with their gene densities and physical sizes. As an example, human 18 and 19 CTs show discrete difference in their positioning in lymphocyte nuclei; the gene-poor human 18 CTs are preferentially located at the nuclear periphery, while the gene-dense human 19 CTs are found in the nuclear interior. Here we investigated by 3D-FISH technique, 1) how radial distribution of CTs is evolutionarily conserved in primates, 2) how degree of relative positioning of CTs is involved in the evolutionary chromosomal translocations? First, we performed 3D-FISH analyses using the human 18 and 19 painting probes on the primate lymphocytes derived from various species, from chimpanzees, gibbons, Old World monkeys (6 species), and New World monkeys (5 species). The results showed that the topology of human 18 and 19 homologous CTs was highly conserved among those primate species. To study within the whole nuclear space, we developed the pooled chromosome paints specific for detecting the peripheral (P) or interior (I) nuclear space. The results by these probes also showed highly evolutionarily conserved even within the gibbon nuclei with highly evolutionary chromosomal rearrangements. Second, we used human chromosome 2p and 2q paints which is considered to be formed after fusing two separate acrocentric chromosomes, present in the great apes and Old World monkeys. Relative positioning of human 2p and 2q homologous CTs in primates showed the frequency with one pair chromosome kissing between 2p and 2q homologs is lower among the Old World monkeys but is higher among the great apes, suggesting that it has tendency with higher frequency of the chromosome kissing between 2p and 2q homologs among the evolutionary closed species from human.

哺乳动物细胞核中的染色体区 (CT) 被高度划分为不同的域，并通过三维荧光原位杂交 (3D-FISH) 技术进行可视化。 CT的径向分布与其基因密度和物理尺寸高度相关。例如，人类 18 和 19 CT 在淋巴细胞核中的定位表现出离散差异；基因贫乏的人类 18 CT 优先位于核外围，而基因密集的人类 19 CT 则位于核内部。在这里，我们通过 3D-FISH 技术研究了：1）CT 的径向分布在灵长类动物中如何进化保守，2）CT 的相对定位程度如何参与进化染色体易位？首先，我们使用人类 18 和 19 绘画探针对来自不同物种（黑猩猩、长臂猿、旧世界猴（6 种）和新世界猴（5 种））的灵长类淋巴细胞进行 3D-FISH 分析。结果表明，人类18和19同源CT的拓扑结构在灵长类动物中高度保守。为了在整个核空间内进行研究，我们开发了专门用于检测外围 (P) 或内部 (I) 核空间的混合染色体涂料。这些探针的结果还表明，即使在具有高度进化染色体重排的长臂猿细胞核内，其在进化上也高度保守。其次，我们使用了人类染色体 2p 和 2q 颜料，这被认为是在融合两个独立的近端着丝粒染色体后形成的，存在于类人猿和旧大陆猴子中。人类2p和2q同源CT在灵长类动物中的相对定位显示，旧世界猴中2p和2q同源物之间一对染色体接吻的频率较低，但在类人猿中较高，这表明在人类进化封闭物种中2p和2q同源物之间的染色体接吻频率具有较高的趋势。

项目成果

期刊论文数量（43）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Relative positioning of human chromosome 2 territories in primates

灵长类动物 2 号染色体区域的相对定位

DOI：
发表时间：
2005
期刊：
影响因子：
0
作者：
Tanabe;H.;Mochizuki;Y.;Amano;M.;Chiba;M.;Nagata;T.
通讯作者：
T.

[Nucleus/Chromosome]2. Chromosome territories

[细胞核/染色体]2。

DOI：
发表时间：
2004
期刊：
Seitai no kagaku Volume 55, No.5
影响因子：
0
作者：
Hideyuki Tanabe;Katrin Kupper;Takafumi Ishida;Michaela Neusser;Hiroshi Mizusawa;Hideyuki Tanabe;田辺秀之;田辺秀之;Hideyuki Tanabe;Hideyuki Tanabe
通讯作者：
Hideyuki Tanabe

Relative positioning of chromosome territories in cell nuclei from human and primate lymphoblastoid cell lines

人类和灵长类淋巴母细胞系细胞核中染色体区域的相对定位