Development of specific gene activation method using polyamide-transcription factor conjugates

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We tried to investigate the regulation of gene expression using synthetic pyrrole-imidazole polyamide. For the investigation of the mechanism of epigenetic regulation, we developed the imaging method for nucleosome and related enzymes. For direct visualization of the nucleosome, we constructed the single molecule observation system using the designed DNA nanostructures. We successfully visualized the sequence-selectivity of functionalized polyamide in the designed DNA nanostructure. We also constructed the single-molecule observation system for the behavior of enzymes using high-speed atomic force microscopy. Using this system, we successfully captured the nucleosome to the DNA nanoscaffold, and observed its behavior at the single-molecule level.