Construction of high-power biofuel cells replaced by fuel cells with platinum catalyst

我们尝试建造大功率生物燃料电池。研究了酶在电极表面的最佳定向、酶的三维固定化以及多个酶在电极上的固定化。我们找到了酶固定电极的最佳处理方法。采用一种新型的嗜热性乙醇脱氢酶，在室温条件下，在80℃条件下可获得10倍以上的高电流密度。通过使用金纳米粒子修饰的电极，获得了极高的静电活性蛋白表面覆盖率，在一个完全填充的单层中获得了270个蛋白质。我们还发现了一种新的醇脱氢酶，它可以催化乙醇的4电子氧化。

We tried to construct high-power biofuel cells. The optimal orientation of enzymes on the electrode surface, three-dimensional immobilization of enzymes, and immobilization of plural enzymes on an electrode were investigated. We found the best treatment of the electrode for enzyme immobilization. Using a novel thermophilic alcohol dehydrogenase, a high current density was observed at 80℃more than 10 times at room temperature. An extremely high surface coverage of electrostatically active protein, 270 proteins in a fully packed monolayer, was obtained through use of the gold nanoparticle-modified electrode. We also found a novel alcohol dehydrogenase which can catalyze 4-electron oxidation of ethanol.