Originally Synthesized Oxy-Redox Sensitive Contrast Substances for in vivo Mapping of Tissue Redox and Oxidant Status in Cancer Using High Field MRI

最初合成的氧氧化还原敏感对比物质，用于使用高场 MRI 体内绘制癌症组织氧化还原和氧化状态

• 批准号: 21611011
• 负责人: ZHELEV Zhivko
• 金额: $ 3.08万
• 依托单位: National Institute of Radiological Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21611011/
• 关键词: MRI; Cancer; Nitroxyl; ニトロキシルラジカル; 酸化還元; ESR; マウス; 酸化還元状態; 大腸がん; 高磁場MRI; フリーラジカル; 電子スピン共鳴; nitroxide; neuroblastoma

I investigated the redox potential, hydrophobicity and T_1 relaxivity of 15 redox sensitive nitroxide probes, using voltametry, EPR spectroscopy and T_1 weighted MRI. The probes were classified in three groups, depending on their hydrophobicity-hydrophilic, slightly hydrophobic and strongly hydrophobic. In each group the probes were classified in two subgroups, depending on its resistance to reduction-rapidly reducing and slowly reducing. Four probes were selected for application in imaging of tissue redox activity in experimental animals using MRI. I established that there is difference in tissue redox activity between early and moderate/terminal stage of cancer development, as well as between tumor core and tumor periphery.

我使用伏安制定，EPR光谱和T_1加权MRI研究了15个氧化还原敏感的氮氧化物探针的氧化还原电位，疏水性和T_1的松弛度。根据探针分为三组，具体取决于其疏水性 - 综合性，略有疏水性和强烈疏水。在每组中，将探针分为两个亚组，具体取决于其对减少降低并缓慢降低的抵抗力。选择了四个探针用于使用MRI在实验动物中组织氧化还原活性的成像。我确定癌症发育的早期和中/末端之间以及肿瘤核心和肿瘤周围之间的组织氧化还原活性差异。

项目成果

EPR signal reduction kinetics of several nitroxyl derivatives in blood in vitro and in vivo

几种硝酰基衍生物在体外和体内血液中的 EPR 信号还原动力学

• 发表时间: 2009
• 期刊: General Physiology and Biophysics 28
• 作者: Zhelev Z.;Matsumoto K.;Gadjeva V.;Aoki I.;et al.
• 通讯作者: et al.

-EPR signal dynamic of several nitroxyl-labeled anticancer drugs in blood in vitro and in vivo

-几种硝酰基标记抗癌药物在体外和体内血液中的EPR信号动态

• 发表时间: 2008
• 作者: Zhelev Z.;K. Matsumoto;V. Gadjeva;A. Zheleva;R. Bakalova;I. Aoki;and K. Anzai
• 通讯作者: and K. Anzai

Nitroxide radicals in brain imaging using T_1 weighted MRI

使用 T_1 加权 MRI 进行脑成像中的氮氧自由基

• 发表时间: 2009
• 作者: Zhelev Z.;Bakalova R.;Matsumoto K.;Aoki I.;et al.
• 通讯作者: et al.

Imaging of cancer by redox-mediated mechanism : a radical diagnostic approach

通过氧化还原介导的机制进行癌症成像：一种根本性的诊断方法

• 发表时间: 2010
• 期刊: Mol Biosyst
• 作者: Zhelev Z;Bakalova R;Aoki I;Gadjeva V;Kanno I
• 通讯作者: Kanno I

-Magnetic resonance imaging of very small disruptions of blood vessel permeability in hypercholesterolemia mice using nitroxide probes

-使用硝基氧探针对高胆固醇血症小鼠血管通透性的非常小的破坏进行磁共振成像

• 发表时间: 2010
• 作者: Bakalova;R.;I. Ishii;Z. Zhelev;S. Shibata;I. Aoki;and M. Kitada
• 通讯作者: and M. Kitada