Investigation and application of new metabolic pathways involved in the synthesis of inosinate and guanylate

研究了肌苷酸和鸟苷酸合成的新代谢途径。以香菇（Lentinula edodes）、养殖鲐鱼（Trachurus japonicus）和萝卜（Raphanus sativus）分别作为蘑菇、鱼类和蔬菜的代表。根据放射性标记前体的原位代谢命运和酶的体外活性，研究了这些食物中嘌呤核糖核苷酸的代谢谱。肌苷酸和鸟苷酸在所有食物中都是通过打捞途径合成的。结果表明，在一定的烹饪、加工和保存条件下，这些食品中存在回收途径。

New metabolic pathways involved in the synthesis of inosinate and guanylate were investigated. Shiitake mushrooms(Lentinula edodes), cultured Japanese jack mackerel(Trachurus japonicus) and Japanese radish(Raphanus sativus) were chosen as the representatives of mushrooms, fishes and vegetables, respectively. Metabolic profiles of purine ribonucleotides in these foods were examined based on the in situ metabolic fate of radio-labelled precursors and the in vitro activities of enzymes. Inosinate and guanylate were synthesized via salvage pathways in all foods. It was suggested that salvage pathways were operative in these foods under certain conditions of cooking, processing and preservation.