Roles of Msx2 in osteoblast proliferation, differentiation and activation for bone remodeling.

到目前为止，还没有明确的报道，Msx2参与成骨细胞分化的机制，这是负责骨重建和再生的正畸治疗。因此，本研究的目的是研究Msx2在小鼠胚胎干细胞成骨分化和骨形成中的作用。将转染Msx2的ES细胞与饲养细胞共培养，检测碱性磷酸酶活性和成骨细胞表型的mRNA水平。还测定了骨结节的矿化值。结果表明，Msx2在控制成骨细胞分化中起关键作用。

To date, there have been no clear reports that Msx2 take part in the mechanism of osteoblast differentiation, which is responsible for the bone remodeling and regeneration in orthodontic treatments. The purpose of the present study, therefore, was to investigate the roles of Msx2 in osteoblast differentiation and bone formation with mouse Embryonic Stem cells. ES cells which transfected with Msx2 were cocultured with feeder cells, and alkaline phosphatase activity and mRNA levels of osteoblastic phenotype were determined for cell differentiation. The value of bone nodules was also determined for mineralization. As a result, it was suggested that Msx2 play a key role in controlling osteoblast differentiation.