Roles of Msx2 in osteoblast proliferation, differentiation and activation for bone remodeling.

Msx2 在成骨细胞增殖、分化和激活骨重塑中的作用。

• 批准号: 21792090
• 负责人: YAMAMOTO Yoshitake
• 金额: $ 2.25万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21792090/
• 关键词: 骨芽細胞; 細胞増殖; 細胞分化; 分化・増殖; 骨改造

To date, there have been no clear reports that Msx2 take part in the mechanism of osteoblast differentiation, which is responsible for the bone remodeling and regeneration in orthodontic treatments. The purpose of the present study, therefore, was to investigate the roles of Msx2 in osteoblast differentiation and bone formation with mouse Embryonic Stem cells. ES cells which transfected with Msx2 were cocultured with feeder cells, and alkaline phosphatase activity and mRNA levels of osteoblastic phenotype were determined for cell differentiation. The value of bone nodules was also determined for mineralization. As a result, it was suggested that Msx2 play a key role in controlling osteoblast differentiation.

到目前为止，还没有明确的报道，Msx2参与成骨细胞分化的机制，这是负责骨重建和再生的正畸治疗。因此，本研究的目的是研究Msx2在小鼠胚胎干细胞成骨分化和骨形成中的作用。将转染Msx2的ES细胞与饲养细胞共培养，检测碱性磷酸酶活性和成骨细胞表型的mRNA水平。还测定了骨结节的矿化值。结果表明，Msx2在控制成骨细胞分化中起关键作用。

项目成果

Roles of Msx2 gene in osteoblast differentiation and osteogenesis

Msx2基因在成骨细胞分化和成骨中的作用

• 发表时间: 2010
• 作者: 山本芳丈(代表);三井薫;小戝健一郎;宮脇正一
• 通讯作者: 宮脇正一