A study of osteoblast differentiation and activation in Msx2 gene-transfer ES/iPS cells.

Msx2 基因转移 ES/iPS 细胞中成骨细胞分化和激活的研究。

• 批准号: 23792438
• 负责人: YAMAMOTO Yoshitake
• 金额: $ 2.33万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23792438/
• 关键词: 骨芽細胞; 細胞分化; 分化・機能活性

There have been no clear reports so far that Msx2 gene take part in the mechanism of osteoblast differentiation, which is responsible for the bone remodeling and regeneration in orthodontic treatments. The purpose of this study, therefore, was to investigate the roles of Msx2 in osteoblast differentiation and osteogenesis with mouse Embryonic Stem (ES) cells and Induced Pluripotent Stem (iPS) cells. ES cells and iPS cells which transfected with Msx2 gene were cocultured with feeder cells, and mRNA levels of osteoblastic phenotype and alkaline phosphatase activity were determined for cell differentiation and activation. The value of bone nodules was also determined for mineralization. As a result, it was suggested that Msx2 play a key role in controlling osteoblast differentiation from ES/iPS cells.

成骨细胞分化是正畸治疗中骨重建和骨再生的重要机制之一，而Msx 2基因是否参与了成骨细胞分化的机制目前尚无明确的报道。因此，本研究的目的是研究Msx 2在成骨细胞分化和成骨小鼠胚胎干细胞（ES）和诱导多能干细胞（iPS）的作用。将转染Msx 2基因的ES细胞和iPS细胞与饲养细胞共培养，检测成骨细胞表型mRNA水平和碱性磷酸酶活性，观察细胞分化和活化情况。还测定了骨结节的矿化值。结果表明，Msx 2在控制ES/iPS细胞向成骨细胞分化中起关键作用。

项目成果

Effects of Msx2 gene on osteogenesis in osteoblasts differentiated from ES/iPS cells

Msx2基因对ES/iPS细胞分化成骨细胞的影响

• 发表时间: 2013
• 作者: 山本芳丈(代表者);三井薫;小戝健一郎;宮脇正一
• 通讯作者: 宮脇正一

骨芽細胞分化および骨形成におけるMsx２の関与

Msx2参与成骨细胞分化和骨形成

• 发表时间: 2011
• 作者: YAMAMOTO Y.;MITSUI K.;KOSAI K.;MIYAWAKI S.
• 通讯作者: MIYAWAKI S.

iPS細胞から分化させた骨芽細胞の骨形成に対するMsx２の及ぼす影響

Msx2对iPS细胞分化成骨细胞骨形成的影响

• 发表时间: 2013
• 作者: 山本芳丈;三井 薫;小戝健一郎;宮脇正一
• 通讯作者: 宮脇正一