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Do the dental factors effect the DNA damage in the mucosal cell?

牙齿因素会影响粘膜细胞的 DNA 损伤吗？

基本信息

批准号：
21792143
负责人：
ISHIKAWA Shigeo
金额：
$ 0.67万
依托单位：
Yamagata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Young Scientists (B)
财政年份：
2009
资助国家：
日本
起止时间：
2009 至 2010
项目状态：
已结题

项目摘要

[Introduction] The micronuclei (MN) assay is one of the most sensitive markers of DNA damage. MN frequency has been used to investigate the genotoxicity of various chemicals. In this study, I evaluated the relationship MN frequency in the mucosal cells and dental factors (ex : periodontitis status, habit of oral hygiene, metals of teeth and resins of teeth,). Our aim was to research the confounding factor for MN test in the mucosal cells and to suggest the more precise MN test in the mucosal cell. [Materials and Method] The study protocol was approved by the Ethics Committee of Yamagata University School of Medicine. The study group consisted of 97 males who had not been exposed to chemicals during their work or leisure time. [Result] The forward stepwise multiple regression analysis showed the effect of age on the MN frequency in the mucosal cells. Age (β=0.45) was only risk factor that significantly increased MN frequency in the mucosal cells in this model. The adjusted coefficient of determination (R^2) in this model was 0.20. Therefor 20.0% of the variability in the MN frequency in the mucosal cells was exppained by age. All of the dental factors in this model were not statistically significant. [Discussion] I revealed the possibility that the dental factors didn't affect the MN frequency in the mucosal cells. Further research is needed with monitoring the metal levels in the serum or scalp hair to investigate the relationship MN test in the mucosal cells and dental factors.

[引言]微核（MN）试验是检测DNA损伤最敏感的指标之一。MN频率已被用于研究各种化学品的遗传毒性。在这项研究中，我评估了粘膜细胞中MN频率与牙科因素（例如：牙周炎状况，口腔卫生习惯，牙齿金属和牙齿树脂）的关系。本研究旨在探讨粘膜细胞微核试验的混杂因素，并提出更准确的粘膜细胞微核试验方法。[材料与方法]研究方案经山形大学医学部伦理委员会批准。研究组由97名男性组成，他们在工作或休闲时间没有接触过化学品。[结果]多元逐步回归分析显示年龄对粘膜细胞微核率有影响。年龄（β=0.45）是该模型中粘膜细胞中MN频率显著增加的唯一危险因素。该模型中调整后的决定系数（R^2）为0.20。因此，20.0%的粘膜细胞MN频率的变异性是由年龄解释的。该模型中的所有牙科因素均无统计学意义。[讨论]揭示了口腔因素不影响粘膜细胞MN频率的可能性。通过监测血清和头发中的金属含量，进一步探讨粘膜细胞MN试验与牙源性因素的关系。