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カルバゾール分解プラスミドpCAR1と宿主染色体との相互作用の可変性・普遍性の解明

阐明咔唑降解质粒 pCAR1 与宿主染色体之间相互作用的变异性和普遍性

基本信息

批准号：
09J05156
负责人：
高橋裕里香
金额：
$ 1.34万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for JSPS Fellows
财政年份：
2009
资助国家：
日本
起止时间：
2009 至 2011
项目状态：
已结题

项目摘要

本研究では,含窒素芳香族化合物カルバゾールの分解プラスミドpCAR1を保持する3種のゲノム既知宿主の転写レベル・形質レベルでの比較によって,プラスミドと宿主染色体間の相互作用が異なる宿主でどのように変化するのか,またその差異を生みだす鍵となる因子は何か,を解析することで,多剤耐性菌の蔓延防止や分解菌による環境修復など実環境中でプラスミドを制御する技術の確立に欠かせない基礎情報を得ることを目的としている.本年度は,昨年度までの転写レベル・表現型レベルの解析によって見出されたpCAR1を保持した宿主に起こる様々な変化が,pCAR1固有のものなのか,それともプラスミド一般に共通のものなのかを明らかにするため,pCAR1とは異なる不和合性群に属するプラスミド2種(多剤耐性プラスミドRP4,ナフタレン分解プラスミドNAH7)をPseudomonas putida KT2440株に保持させ,プラスミド保持株と非保持株のトランスクリプトーム比較を行った.その結果,プラスミドを保持した際に転写変動する遺伝子の数はRP4・NAH7のほうがpCAR1よりも多いこと,pCAR1では抑制される遺伝子よりも誘導される遺伝子の方が多いのに対しRP4・NAH7では抑制される遺伝子のほうが多いこと,が明らかになった.しかし興味深い点として,pyrroloquinoline quinone(PQQ)合成遺伝子とPQQ含有酵素遺伝子を含む1領域が3種のプラスミド保持株で共通に転写誘導されており,全く種類の異なるプラスミドでも宿主に共通の反応を引き起こすことが示された。

In this study, aromatic compounds containing asphyxiate were isolated and analyzed. In this study, aromatic compounds containing asphyxiant were isolated and analyzed. In this study, aromatic compounds containing asphyxiant were isolated, the decomposition of asphyxiant aromatic compounds, the decomposition of asphyxiant aromatic compounds, the maintenance of three kinds of aromatic compounds, the analysis of the interaction between host chromosomes, the biodegradation of asphyxiate aromatic compounds, the decomposition of asphyxiate aromatic compounds, the decomposition of asphyxiate aromatic compounds, the retention of asphyxiate aromatic compounds, the degradation of asphyxiate aromatic compounds, the degradation of asphyxiate aromatic compounds, the reduction of asphyxiant aromatic compounds, the reduction of asphyxiant aromatic compounds, the degradation of asphyxiant aromatic compounds, the reduction of asphyxiate aromatic compounds, the decomposition of asphyxiant aromatic compounds, the maintenance of three kinds The spread of multi-resistant bacteria to prevent the spread of decomposing bacteria in the environment to prevent the spread of multi-resistant bacteria to prevent the spread of multi-resistant bacteria to prevent the spread of multi-resistant bacteria to prevent the spread of multi-resistant bacteria. This year, last year, we wrote about this year, and the tables were used to analyze the data. This year, we found that the pCAR1 was maintained on the host computer, and that the pCAR1 was in common use. In general, there was a general agreement that the discordance group belonged to two categories (multiple endurance RP4). The Pseudomonas putida KT2440 plant is not a maintainer. The non-maintainer plant is not the maintainer plant. The results show that you need to maintain the number of messages that you can write, RP4, NAH7, pCAR1, and pCAR1. If you want to do so, you will need to know that you will not be able to do so, and that you will know that you will not be able to do so. Pyrroloquinoline quinone (PQQ) synthetic spores PQQ contains enzymes that contain enzymes that are common to the maintenance plants in the field of 1, and are common to all hosts in the field.