Fundamental process design for immobilized enzyme reaction in a gas/solid bioreactor

在气固生物反应器中，研究了固定化酮还原酶(醇脱氢酶)催化酮合成手性醇的反应。作为连续反应工艺的基础设计，优化了固定化酶制备条件与降压度、缓冲液pH、缓冲液浓度的函数关系，以及反应条件与水浓度、温度的函数关系。并对气相反应的最佳反应体系和酶的来源进行了研究。

The chiral alcohol production from ketone catalyzed by immobilized ketoreductases (alcohol dehydrogenases) has been investigated in a gas-solid bioreactor. As fundamental design of continuous reaction process, the conditions for immobilized enzyme preparation as functions of the reduced pressure degree, the buffer pH as well as the buffer concentration and for reaction as functions of the water concentration and the temperature were optimized. Also, the optimal reaction systems and enzyme sources for the gas phase reaction were investigated.