Development of a novel cell source for regenerative medicine in elderly patients

我们研究了成熟的脂肪细胞来源的去分化脂肪（DFAT）细胞对成骨和血管生成分化的有效诱导，这些细胞是从不同年龄的患者的脂肪组织中制备的。结果表明，骨髓脂肪细胞制备的DFAT细胞表现出较高的成骨潜能，与供体年龄无关。低氧（1%）条件下培养的DFAT细胞培养基显示出较强的血管生成活性。DFAT细胞移植可增加骨质疏松症动物模型的骨密度。在小鼠后肢缺血模型中，与外周血单核细胞移植小鼠相比，DFAT细胞移植小鼠的血流量增加。这些结果表明，DFAT细胞可能是老年骨质疏松症和外周动脉疾病患者细胞基础治疗的一个有吸引力的细胞来源。

We examined efficient induction of osteogenic and angiogenic differentiation in mature adipocyte-derived dedifferentiated fat (DFAT) cells, which were prepared from adipose tissue of patients of various ages. The results showed that DFAT cells prepared form bone marrow adipocytes exhibited high osteogenic potential regardless of donor age. Conditioned media from cultured DFAT cells under low (1%) oxygen condition showed strong angiogenic activity. DFAT cell transplantation increased bone density in an animal model of osteoporosis induced by overiectomy. In a mouse ischemic hind limb model, an increase in blood flow was observed in DFAT cell-transplanted mice, compared to that in peripheral blood mononuclear cell-transplanted mice. These results suggest that DFAT cells may be an attractive cell source for cell-based therapy in elderly patients with osteoporosis and peripheral artery disease.