Development of a novel amino acid solubility propensity scale for the calculation of polypeptide solubility
开发用于计算多肽溶解度的新型氨基酸溶解度倾向量表
基本信息
- 批准号:21300110
- 负责人:
- 金额:$ 11.65万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:2009
- 资助国家:日本
- 起止时间:2009 至 2011
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Protein solubility is usually characterized in terms of a hydrophobicity scale, which refers to the free energy of transfer of a molecule from an aqueous to a nonpolar solution and is not a "solubility propensity scale" per se. Here, we measured the effects of short poly-amino-acid tags(guests) on the solubility of a host protein, a simplified bovine pancreatic trypsin inhibitor(BPTI), to which they were fused at the C-terminus. We analyzed 10 amino acid types, representing the full range of biophysical properties(acidic, basic, polar, and hydrophobic). As anticipated, positively charged residues significantly increased the solubility of the model protein, at both pH 4. 7 and 8. 7, whereas very hydrophobic poly-Ile markedly reduced the solubility of BPTI. Furthermore, to ensure that the measured solubility values were context independent and could provide a genuine "solubility propensity scale", we confirmed that the tags remained independent of the structure, thermal stability, and biochemical activity of the host protein. These observations suggest that this approach is valuable for measuring the solubility propensities of amino acids, which could eventually allow the calculation of a polypeptide's relative solubility from its amino acid sequence.
蛋白质溶解度通常以疏水性尺度来表征,其是指分子从水溶液转移到非极性溶液的自由能,而不是“溶解度倾向尺度”本身。在这里,我们测量了短的聚氨基酸标签(客人)的宿主蛋白质,一个简化的牛胰蛋白酶抑制剂(BPTI),他们在C-末端融合的溶解度的影响。我们分析了10种氨基酸类型,代表了生物物理特性的全部范围(酸性,碱性,极性和疏水性)。正如预期的那样,带正电荷的残基显著增加了模型蛋白质的溶解度,在两种pH 4下。7和8 7,而非常疏水的poly-Ile显著降低BPTI的溶解度。此外,为了确保所测量的溶解度值是上下文无关的并且可以提供真正的“溶解度倾向量表”,我们确认标签保持与宿主蛋白的结构、热稳定性和生物化学活性无关。这些观察结果表明,这种方法对于测量氨基酸的溶解度倾向是有价值的,这最终可以允许从其氨基酸序列计算多肽的相对溶解度。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
MOBILITY OF WATER MOLECULES INTO GFP INTERIOR AND THEIR EFFECTS ON THE THERMAL STABILITY
水分子进入 GFP 内部的迁移率及其对热稳定性的影响
- DOI:
- 发表时间:2009
- 期刊:
- 影响因子:0
- 作者:秋山沙織;小林貴幸;上岡哲矢;末永敦;泰地真弘人;黒田裕
- 通讯作者:黒田裕
難結晶化タンパク質変異体に対するヘテロシーディング法
难结晶蛋白突变体的异种播种方法
- DOI:
- 发表时间:2011
- 期刊:
- 影响因子:0
- 作者:小林慶;Mohammad M Islam;野口恵一;養王田正文;黒田裕
- 通讯作者:黒田裕
Application of 3D-profile to Comparative Studies of Profiles
3D轮廓在轮廓比较研究中的应用
- DOI:
- 发表时间:2010
- 期刊:
- 影响因子:0
- 作者:Oda;H.;Ota;M.;Sato;T.;Toh;H.
- 通讯作者:H.
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
KURODA Yutaka其他文献
KURODA Yutaka的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('KURODA Yutaka', 18)}}的其他基金
Novel screening protocol for multi-SS bond proteins using SEP tags and its application to the development of a minimal Luciferase
使用 SEP 标签的多 SS 键蛋白的新型筛选方案及其在最小荧光素酶开发中的应用
- 批准号:
23651213 - 财政年份:2011
- 资助金额:
$ 11.65万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Construction of a support vector machine (SVM) for accurately detecting domain boundaries
构建支持向量机(SVM)以准确检测域边界
- 批准号:
18500225 - 财政年份:2006
- 资助金额:
$ 11.65万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Construction of a neural network for detecting novel domains from amino acid sequence information only
构建仅从氨基酸序列信息检测新结构域的神经网络
- 批准号:
16500189 - 财政年份:2004
- 资助金额:
$ 11.65万 - 项目类别:
Grant-in-Aid for Scientific Research (C)