Development of a New Pin-point Treatment of Cancer Cells Using Hydrogen Storage Alloy as Hydrogen Supplier

使用储氢合金作为氢供应商开发一种新的癌细胞精确治疗方法

• 批准号: 21300170
• 负责人: KAGAWA Akio
• 金额: $ 11.98万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21300170/
• 关键词: 金属物性; 細胞・組織; 水素; 癌; 水素貯蔵合金; パラジウム; ニッケル; 正常細胞; 癌細胞; 水素ラジカル; スーパーオキシド

To develop a pin-point treatment of cancer cells, hydrogen discharging behavior of hydrogen storage alloy(HSA) and the effect of hydrogen discharged from the HSA with different alloy composition and sample shape on biological cells have been studied. The results obtained are summarized as follows. 1) The amount of gaseous hydrogen and hydrogen ions as well as hydrogen radicals varied depending upon alloy composition. It is, therefore, possible to control the hydrogen discharging behavior by adjusting the alloy composition. 2) From the observation of the probability-of-survival(POS) measurement, a reduction in the POS was observed in cancer cells and a part of normal cell, and it was found that the influence of discharged hydrogen depends on the kind of cells. 3) A selective cancer cell death was observed in the region of approximately 2~ 2. 5 mm outside the samples, independent of sample shape. 4) From the ESR experiment and the detection of hydrogen peroxide using fluorescent regent, hydrogen radical, hydroxyl radical and hydrogen peroxide were detected and it was suggested that these hydrogen species would affect to cancer cell death.

为开发肿瘤细胞的定点治疗方法，研究了贮氢合金（HSA）的放氢行为，以及不同合金成分和试样形状的HSA放氢对生物细胞的影响。所得结果总结如下。1)气态氢和氢离子以及氢自由基的量根据合金成分而变化。因此，可以通过调节合金成分来控制氢释放行为。2)从存活概率（POS）测量的观察中，在癌细胞和一部分正常细胞中观察到POS的减少，并且发现释放的氢的影响取决于细胞的种类。3)在约2~ 2 μ mol/L的范围内观察到选择性癌细胞死亡。样品外5 mm，与样品形状无关。4)通过ESR实验和荧光试剂检测过氧化氢，检测到了氢自由基、羟基自由基和过氧化氢，提示这些氢物种对癌细胞的死亡有影响。

项目成果

水素吸蔵合金からの放出水素が生体細胞に及ぼす影響

储氢合金释放的氢气对活细胞的影响

• 发表时间: 2009
• 作者: 梅木宣明;水本将之;大貝猛;香川明男
• 通讯作者: 香川明男

Influence of Hydrogen Discharged from Palladium Base Hydrogen Storage Alloys on Cancer Cells

• DOI: 10.4028/www.scientific.net/msf.706-709.520
• 发表时间: 2012-01
• 期刊: Materials Science Forum
• 作者: A. Kagawa;K. Katsura;M. Mizumoto;Yutaka Tagawa;Yoichi Masiko

Bending and Rotation Movement Control of a Novel Actuator Utilizing Hydrogen Storage Alloys

利用储氢合金的新型执行器的弯曲和旋转运动控制

• 发表时间: 2011
• 期刊: Advanced Materials Research
• 作者: A. Nakai;M. Mizumoto and A. Kagawa
• 通讯作者: M. Mizumoto and A. Kagawa

生体細胞への水素放出効果に及ぼす水素吸蔵合金の形状の影響

储氢合金形状对活细胞释氢效果的影响

• 发表时间: 2010
• 作者: 桂啓志;水本将之;大貝猛;香川明男;田川泰
• 通讯作者: 田川泰

生体細胞に及ぼす水素吸蔵合金からの水素放出の影響

储氢合金释氢对活细胞的影响

• 发表时间: 2011
• 作者: 松崎真実;水本将之;香川明男;大貝猛
• 通讯作者: 大貝猛