Development of a New Pin-point Treatment of Cancer Cells Using Hydrogen Storage Alloy as Hydrogen Supplier

为了建立肿瘤细胞的定点治疗方法，研究了储氢合金（HSA）的放氢行为，以及不同合金成分和样品形状的储氢合金放氢对生物细胞的影响。所得结果总结如下：1)气态氢、氢离子和氢自由基的含量因合金成分的不同而不同。因此，可以通过调整合金成分来控制氢放电行为。2)通过观察存活概率（POS）测量，发现癌细胞和部分正常细胞的POS值均有所降低，且放电氢气对存活概率的影响与细胞种类有关。3)在约2~ 2的范围内观察到癌细胞的选择性死亡。样品外5mm，与样品形状无关。4)通过ESR实验和荧光试剂检测过氧化氢，检测出氢自由基、羟基自由基和过氧化氢，并提示这些氢对癌细胞死亡有影响。

To develop a pin-point treatment of cancer cells, hydrogen discharging behavior of hydrogen storage alloy(HSA) and the effect of hydrogen discharged from the HSA with different alloy composition and sample shape on biological cells have been studied. The results obtained are summarized as follows. 1) The amount of gaseous hydrogen and hydrogen ions as well as hydrogen radicals varied depending upon alloy composition. It is, therefore, possible to control the hydrogen discharging behavior by adjusting the alloy composition. 2) From the observation of the probability-of-survival(POS) measurement, a reduction in the POS was observed in cancer cells and a part of normal cell, and it was found that the influence of discharged hydrogen depends on the kind of cells. 3) A selective cancer cell death was observed in the region of approximately 2~ 2. 5 mm outside the samples, independent of sample shape. 4) From the ESR experiment and the detection of hydrogen peroxide using fluorescent regent, hydrogen radical, hydroxyl radical and hydrogen peroxide were detected and it was suggested that these hydrogen species would affect to cancer cell death.