Control of intranuclear disposition of DNA for gene therapy

为了实现有效和持久的转基因表达的控制核内处置，我们插入DNA序列，调节结合组蛋白到质粒DNA，组蛋白-质粒DNA复合物引入细胞，并开发了一个质粒系统，招募转录因子。我们发现转基因表达效率提高，并维持一周。此外，我们发现，基因校正的尾双链体是一个有用的候选基因治疗。

To achieve efficient and durable transgene expression by controlled intranuclear disposition, we inserted DNA sequences that modulate binding to histones into plasmid DNAs, introduced histone-plasmid DNA complexes into cells, and developed a plasmid system that recruited transcription factors. We found that transgene expression was improved in efficiency and was maintained for a week. Moreover, we revealed that gene correction by tailed duplexes was a useful candidate in gene therapy.