Development of Therapeutic Drugs Targeting Cancer with Bio-Nanoparticle Encapsulating Anti-Cancer Reagents

利用生物纳米颗粒封装抗癌试剂开发针对癌症的治疗药物

• 批准号: 21300179
• 负责人: SENO Masaharu
• 金额: $ 11.65万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21300179/
• 关键词: 制がん剤; ErbB2; CD44; DDSキャリア; 分子標的; 細胞表面マーカー; DNAマイクロアレイ; 球面自己組織化マップ

We tried to establish drug delivery systems, targeting cancer cells specifically, using bio-nano-particle composed of liposome and/or proteins as a carrier of anti-cancer reagents. Multivalent design of the carrier to present antibodies or ligands with high affinity to cell surface molecules was found to stimulate endocytosis of the particles. The targeting with the antibodies and ligands made the targeting efficient showing the incubation time of particle with the target cells clearly shorter. Furthermore, the particle escaped from phagocytic macrophages when lipid component was optimized.

我们试图建立专门针对癌细胞的药物递送系统，使用由脂质体和/或蛋白质组成的生物纳米颗粒作为抗癌试剂的载体。发现对细胞表面分子具有高亲和力的抗体或配体的载体多价设计可以刺激颗粒的内吞作用。抗体和配体的靶向使得靶向有效，显示颗粒与靶细胞的孵育时间明显更短。此外，当脂质成分被优化时，颗粒从吞噬巨噬细胞中逃脱。

项目成果

Enhanced internalization of ErbB2 in SK-BR-3 cells with multivalent forms of artificial ligand

使用多价形式的人工配体增强 SK-BR-3 细胞中 ErbB2 的内化

• 发表时间: 2011
• 期刊: J Cell Mol Med.
• 作者: Vaidyanath A;Hashizume T;Nagaoka T;Takeyasu N;Satoh H;Chen L;Wang J;Kasai T;Kudoh T;Satoh A;Fu L;Seno M
• 通讯作者: Seno M

Construction of a high-efficiency multi-site-directed mutagenesis.

高效多位点定向诱变的构建。

• 发表时间: 2011
• 期刊: African J Biotechnol
• 作者: Tan H;Li Y;Chen L;Kasai T;Seno M.
• 通讯作者: Seno M.

Development of Efficient Method for the Synthesis of Polycyclic Hetero Aromatic Compounds Utilizing Intramolecular Anionic Diels-Alder Reactions

利用分子内阴离子狄尔斯-阿尔德反应合成多环杂芳族化合物的有效方法的开发

• 发表时间: 2010
• 作者: Kensaku Mori;Masahiro Oda;Tomohiko Egusa;Zhengang Jiang;Takayuki Kitasaka;Michitaka Fujiwara and Kazunari Misawa;工藤孝幸
• 通讯作者: 工藤孝幸

Self Organizing Maps - Applications and Novel Algorithm Design

• DOI: 10.5772/566
• 发表时间: 2011-01
• 作者: J. Mwasiagi

The conformational polymorphism of green fluorescent protein

绿色荧光蛋白的构象多态性

• 发表时间: 2012
• 期刊: Molecular Biology
• 影响因子: 1.2
• 作者: Tan H;Li YG;Chen L;Kudoh T;Kasai T;Seno M
• 通讯作者: Seno M