Elucidations of the mechanisms for the formation of cholesterol-ozonolysis products in inflamed tissues, their biological activities and involvement in disease development

我们开发了一种高度灵敏和特异的方法来分析动脉粥样硬化和胆固醇臭解产物。利用这种方法，我们已经报道了炎症组织中的人中性粒细胞可以产生一种臭氧样氧化剂(S)，这种氧化剂可以氧化胆固醇生成动脉粥样硬化。由于动脉粥样硬化通过抑制一氧化氮合酶来损害重要信号分子一氧化氮的形成，并对包括神经细胞在内的各种细胞产生强大的细胞毒性作用，这些可能是阿尔茨海默病和其他疾病的病原体。

We have developed a highly sensitive and specific method to analyze atheronals, cholesterol ozonolysis products. Using this method, we have reported that human neutrophils in inflamed tissues can produce an ozone-like oxidant(s) which can oxidize cholesterol to generate atheronals. Because atheronals impair the formation of an important signal molecule, nitric oxide, by inhibiting nitric oxide synthases and also exert potent cytotoxic effects against a variety of cells including neuronal cells, these could be causative agents for Alzheimer's disease and others.