Amyloid beta-42-mimotope displaying M13 bacteriophage for vaccine vehicle of Alzheimer's disease

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Bacteriophage evokes strong antibody production by means of innate immunity. Recently, we have established B6 mimotope peptide(B6-C15) which mimics the structure of amyloid・42(A・) fibril using human single-chain Fv(B6) specific to A・42 fibril. This mimotope peptide binds to A・42 oligomers and inhibits formation of A・42 fibril in vitro. We investigated whether M13 bacteriophage displaying B6-mimotope peptide(B6-C15-phage) could produce A・42 fibril specific antibody response in mice. When mice were immunized i. p. with B6-C15-phage in PBS solution, they produced anti-A・42 fibril specific IgG response in two weeks. This finding suggested a promising vaccination strategy for immunotherapy of Alzheimer's disease without the involvement of A・42-specific T cell immunity(BBRC 402 : 19-22, 2010, and J. Neuroimmunol. 236 : 27-38, 2011).