Study on molecular targeting using phage dispky library

在这项研究中，我们研究了利用噬菌体展示文库的分子靶向，特别是对两种肽模拟物和三种人单链病毒抗体作为人类治疗试剂的候选物进行了表征。1)建立用于治疗目的的肽拮抗剂和人抗体2)开发低分子形式的人抗体3)通过抗体工程开发新的生物功能分子关于1)：我们建立了抗朊病毒、抗淀粉样蛋白和抗il -18抗体，这些抗体可用于人类的诊断和治疗方法。关于2)：在抗β淀粉样蛋白的情况下，我们成功建立了具有抑制淀粉样蛋白纤维化活性的β淀粉样蛋白特异性VI片段。关于3)：我们尝试在杂交瘤中利用AID（激活诱导胞苷脱氨酶）活性来研究scFv亲和成熟的方法。本研究采用腺病毒载体、逆转录病毒载体、普通电泳DNA转染或脂质体转染等多种基因转染方法，考察了AID在杂交瘤中的转染效率

In this study, we studied about the molecular targeting using phage display library, particularly, on the characterization of two peptide mimics and three human scFv antibodies as candidates for human therapeutic reagents.1)Establishment of peptide antagonists and human antibodies for therapeutic purpose2)Development of low molecular forms of human antibodies3)Development of novel biofunctional molecules by antibody engineeringRegarding 1) : we established anti-prion scFv, anti-amyloid beta scFv and anti-IL-18 antibodies which are useful for human diagnostic and therapeutic approaches.Regarding 2) : In the case of anti-amyloid beta, we successfully established the amyloid beta-specific VI fragment with the inhibitory activity of amyloid fiblilization.Regarding 3) : we attempted to study the methodology for the affinity maturation of scFv using AID (activation-induced cytidine deaminase) activity in hybridomas. In this study, we examined the transfection efficiency of AID to hybridomas using various gene transfection methods such as adenovirus vector, retrovirus vector, usual electrophoretical DNA transfection or lipofectamine-transfection