Study on molecular targeting using phage dispky library

在这项研究中，我们利用噬菌体展示文库对分子靶向进行了研究，特别是对两种多肽模拟物和三种人源单链抗体作为人类治疗试剂的候选进行了表征。1)建立了用于治疗目的的多肽拮抗剂和人源抗体2)开发了低分子形式的人抗体3)通过抗体工程开发了新的生物功能分子1)：我们建立了对人类诊断和治疗有用的抗Prion scFv、抗淀粉样β单链抗体和抗IL-18抗体。我们成功地构建了具有抑制淀粉样纤化活性的淀粉样β蛋白特异性VI片段。3)：我们尝试在杂交瘤中利用激活诱导胞苷脱氨酶(AID)活性来研究单链抗体亲和力成熟的方法学。在本研究中，我们用不同的基因转染法，如腺病毒载体、逆转录病毒载体、常规的电泳法dna转染法和脂质体转染法，检测了aid对杂交瘤的转染率。

In this study, we studied about the molecular targeting using phage display library, particularly, on the characterization of two peptide mimics and three human scFv antibodies as candidates for human therapeutic reagents.1)Establishment of peptide antagonists and human antibodies for therapeutic purpose2)Development of low molecular forms of human antibodies3)Development of novel biofunctional molecules by antibody engineeringRegarding 1) : we established anti-prion scFv, anti-amyloid beta scFv and anti-IL-18 antibodies which are useful for human diagnostic and therapeutic approaches.Regarding 2) : In the case of anti-amyloid beta, we successfully established the amyloid beta-specific VI fragment with the inhibitory activity of amyloid fiblilization.Regarding 3) : we attempted to study the methodology for the affinity maturation of scFv using AID (activation-induced cytidine deaminase) activity in hybridomas. In this study, we examined the transfection efficiency of AID to hybridomas using various gene transfection methods such as adenovirus vector, retrovirus vector, usual electrophoretical DNA transfection or lipofectamine-transfection