Single Cell Deformability Measurement Based on the Combined Technology of Cell Flow Control and its Electric Detection

本研究提出了一种创新的方法来有效检测红细胞（RBC）作为典型单细胞的可变形性特征差异，并将其应用于微流体实验中，该方法使用了一对精心设计并安装在微通道表面的反型膜电极。在微通道中引入了高剪切速率流，RBC随其健康状况而改变其形状。电极监测了与RBC、DI的变形指数相对应的电resistanceΔR信号模式。结果发现，DI与maximumδofΔR峰的半峰全宽密切相关，而与峰值本身无关。

An innovative method to effectively detect a characteristic difference in the deformability of red blood cell(RBC) as a typical example of single cells has been proposed in this study and applied to micro-fluidic experiments using a pair of counter-type membrane electrodes carefully designed and mounted on the surface of a microchannel. The flows of high shear rate with which an RBC changes its shape depending on its health condition were introduced in the microchannel. The electrodes monitored the signal patterns of electric resistanceΔR which corresponds to the deformability index of RBC, DI. It was found that DI was closely correlated with the full width of half maximumδofΔR peak, rather than the peak value itself.