Single Cell Deformability Measurement Based on the Combined Technology of Cell Flow Control and its Electric Detection

基于细胞流动控制及其电检测组合技术的单细胞变形能力测量

• 批准号: 21360095
• 负责人: NAKABE Kazuyoshi
• 金额: $ 12.15万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21360095/
• 关键词: 物質輸送; 細胞変形能; マイクロ流路; 剪断応力; 誘電分光; 電場強度分布; 速度分布; 可視化技術; 電場分布

An innovative method to effectively detect a characteristic difference in the deformability of red blood cell(RBC) as a typical example of single cells has been proposed in this study and applied to micro-fluidic experiments using a pair of counter-type membrane electrodes carefully designed and mounted on the surface of a microchannel. The flows of high shear rate with which an RBC changes its shape depending on its health condition were introduced in the microchannel. The electrodes monitored the signal patterns of electric resistanceΔR which corresponds to the deformability index of RBC, DI. It was found that DI was closely correlated with the full width of half maximumδofΔR peak, rather than the peak value itself.

以单细胞为例，提出了一种有效检测红细胞变形性特征差异的新方法，并将其应用于微流控实验中，使用了精心设计和安装在微通道表面的一对对应型膜电极。在微通道中引入了高剪切率的流动，根据红细胞的健康状况改变其形状。电极监测与红细胞变形性指数DI相对应的电阻ΔR的信号模式。结果发现，DI与δR峰半峰全宽密切相关，而与ΔR峰本身无关。

项目成果

Numerical Study on Flow and Heat Transfer Characteristics of Peristaltic Pump

蠕动泵流动与传热特性的数值研究

• 发表时间: 2009
• 作者: R.Kuroki;K.Tatsumi;M.Nakamura;K.Nakabe
• 通讯作者: K.Nakabe

Numerical and Experimental Study on Measurement of a Single Red Blood Cell Deformability using a Microchannel and Electric Sensors

使用微通道和电传感器测量单个红细胞变形能力的数值和实验研究

• 发表时间: 2011
• 作者: R.Fujiwara;K.Tatsumi;Y.Katsumoto;K.Nakabe
• 通讯作者: K.Nakabe

Numerical Model for Micro particle and Lymphocyte Motions in Dieletrophoretic Manipulation Device, Proc.

介电电泳操纵装置中微粒和淋巴细胞运动的数值模型，Proc。

• 发表时间: 2011
• 作者: K. Tatsumi;K. Imajou;H. Shintani;Y. Katsumoto and K. Nakabe
• 通讯作者: Y. Katsumoto and K. Nakabe

一様磁場下の流体中における赤血球運動に関する数値解析モデルの開発

均匀磁场下红细胞在流体中运动的数值分析模型的建立

• 发表时间: 2011
• 作者: 荒川智樹;小森優輝;巽和也;中部主敬
• 通讯作者: 中部主敬

Numerical Modeling of Red Blood Cell Suspended in a Channel with Uniform Magnetic Field

悬浮在均匀磁场通道中的红细胞的数值模拟

• 发表时间: 2011
• 作者: K.Tatsumi;R.Kuroki;K.Nishitani;T.Arakawa;K.Nakabe
• 通讯作者: K.Nakabe