Evaluation of nitrogen loading and its ecological consequences in the coastal marine ecosystem using compound-specific isotope analysis

利用化合物特异性同位素分析评估沿海海洋生态系统的氮负荷及其生态后果

• 批准号: 21370008
• 负责人: MIYAJIMA Toshihiro
• 金额: $ 11.15万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21370008/
• 关键词: 海洋生態; 環境; 窒素負荷; アミノ酸; 安定同位体比; 大気降下物; 共生系; サンゴ礁; 物質循環

Compound-specific δ15N analysis has been employed to investigate ecological effects of anthropogenic nitrogen loading to the coastal marine ecosystems, particularly coral reefs. Spatial change in δ15N along environmental gradient of N loading was compared between the traditional bulk-δ15N and the amino acid δ15N measurements for both the host corals and the zooxanthellae. The mechanism for internal N exchange between the host coral and the zooxanthellae was investigated by comparing the amino acid δ15N pattern among corals incubated under different light and feeding conditions. To evaluate the detrital food chain using the amino acid δ15N, bacteria were incubated under several different nutritional conditions and compared for the amino acid δ15N pattern. We also evaluated the influence of atmospheric N deposition on coral reefs, which has been almost overlooked in previous works. The origin and the transport pathway were estimated using δ15N and δ18O of rainwater nitrate, δ18O and δ2H of meteoric water, and the backward trajectory analysis.

已经采用了化合物特异性Δ15N分析来研究人为氮对沿海海洋生态系统（尤其是珊瑚礁）的生态影响。比较了传统的散装 - Δ15N和氨基酸Δ15N测量值和Zooxanthellae的氨基酸δ15N和氨基酸Δ15N的测量，Δ15N的空间变化沿N负载的环境梯度进行了比较。通过比较在不同的光和喂食条件下孵育的珊瑚中的氨基酸δ15N模式进行了研究。为了使用氨基酸δ15N评估碎屑食物链，在几种不同的营养条件下孵育细菌，并比较氨基酸Δ15N模式。我们还评估了大气N沉积对珊瑚礁的影响，珊瑚礁几乎在以前的工作中被忽略了。使用硝酸盐，Δ18O和流星水的δ18O和Δ2H的δ15N和Δ18O估算了原点和传输途径，以及向后的轨迹分析。

项目成果

Seasonal and regional variability in nutrient inputs to sea surface by precipitation

降水对海面营养物质输入的季节性和区域变化

• 发表时间: 2012
• 作者: Ito Katsura;Fukuda Tatsuya;Hayakawa Hiroshi;Arakawa Ryo;Saito Yutaka;中井 泉;Toshihiro Miyajima
• 通讯作者: Toshihiro Miyajima

Controlling factors of seasonal variation in the nitrogen isotope ratio of nitrate in a eutrophic coastal environment

• DOI: 10.1016/j.ecss.2009.08.006
• 发表时间: 2009-11
• 期刊: Estuarine Coastal and Shelf Science
• 影响因子: 2.8
• 作者: R. Sugimoto;A. Kasai;T. Miyajima;K. Fujita

Stable isotope composition of nitrogen (δ^15N) as a tool for investigating nitrogen cycling in coral reef ecosystems.

氮的稳定同位素组成 (δ^15N) 作为研究珊瑚礁生态系统氮循环的工具。

• 发表时间: 2010
• 作者: Miyajima T;Umezawa Y
• 通讯作者: Umezawa Y

Determi- nation of nitrogen sources for scleratinian corals by compound-specific isotope ratios of amino acids

通过氨基酸的化合物特异性同位素比测定巩膜珊瑚的氮源

• 发表时间: 2010
• 作者: Nakabayashi;J.;and Sasaki;A.;Fujii T
• 通讯作者: Fujii T

Bacterial decomposition of coral mucus as evaluated by long-term and quantitative observation

• DOI: 10.1007/s00338-011-0729-3
• 发表时间: 2011-02
• 期刊: Coral Reefs
• 影响因子: 3.5
• 作者: Yasuaki Tanaka;H. Ogawa;T. Miyajima