Development of effective promoters and efficient systems for gene function analysis in insects

开发昆虫基因功能分析的有效启动子和高效系统

• 批准号: 21380041
• 负责人: SEZUTSU Hideki
• 金额: $ 11.56万
• 依托单位: National Institute of Agrobiological Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21380041/
• 关键词: 昆虫分子生物学; トランスジェニック昆虫; プロモーター; カイコ; ナミテントウ; キイロショウジョウバエ; カブラハバチ; エンハンサートラップ; 部位特異的組換え; 人工プロモーター; トランスポゾン

Systems to efficiently analyze gene function in insects were developed. Site-specific recombination works in silkworm and the system was found to be applicable for the clonal analysis and enhancer trapping. Gene editing utilizing artificial nucleases becomes possible. A promoter of baculovirus promoted expression of transgenes in some species belonging to different orders suggesting its utility across the insects.

开发了有效分析昆虫基因功能的系统。该系统可用于家蚕的克隆分析和增强子捕获。利用人工核酸酶进行基因编辑成为可能。杆状病毒的启动子促进转基因在属于不同目的的一些物种中的表达，表明其在昆虫中的实用性。

项目成果

A CD36-related Transmembrane Protein Is Coordinated with an Intracellular Lipid-binding Protein in Selective Carotenoid Transport for Cocoon Coloration

• DOI: 10.1074/jbc.m109.074435
• 发表时间: 2010-03-05
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Sakudoh, Takashi;Iizuka, Tetsuya;Tsuchida, Kozo
• 通讯作者: Tsuchida, Kozo

Transgenic tools for members of the genus Drosophila with sequenced genomes

• DOI: 10.4161/fly.4.4.13304
• 发表时间: 2010-10-01
• 期刊: FLY
• 影响因子: 1.2
• 作者: Holtzman, Stacy;Miller, David;Kaufman, Thomas
• 通讯作者: Kaufman, Thomas

A single sex pheromone receptor determines chemical response specificity of sexual behavior in the silkmoth Bombyx mori.

• DOI: 10.1371/journal.pgen.1002115
• 发表时间: 2011-06
• 期刊: PLoS genetics
• 影响因子: 4.5
• 作者: Sakurai T;Mitsuno H;Haupt SS;Uchino K;Yokohari F;Nishioka T;Kobayashi I;Sezutsu H;Tamura T;Kanzaki R
• 通讯作者: Kanzaki R

Transgenic silkworm as a tool for functional analysis of insect genes and for bioreactor

转基因蚕作为昆虫基因功能分析和生物反应器的工具

• 发表时间: 2011
• 作者: Tamura;T. et al.
• 通讯作者: T. et al.

Construction of an enhancer trap system and Bombyx Trap DataBase for the analysis of gene function in silkworm Bombyx mori

家蚕基因功能分析的增强子诱捕系统和家蚕诱捕数据库的构建

• 发表时间: 2009
• 作者: Sezutsu H.;et al.
• 通讯作者: et al.