A single sex pheromone receptor determines chemical response specificity of sexual behavior in the silkmoth Bombyx mori.
A single sex pheromone receptor determines chemical response specificity of sexual behavior in the silkmoth Bombyx mori.
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DOI：
10.1371/journal.pgen.1002115
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发表时间：
2011-06
期刊：
影响因子：
4.5
通讯作者：
Kanzaki R
中科院分区：
文献类型：
--
作者：
Sakurai T;Mitsuno H;Haupt SS;Uchino K;Yokohari F;Nishioka T;Kobayashi I;Sezutsu H;Tamura T;Kanzaki R
在昆虫和其他动物中，异性个体之间的种内交流部分是由称为性信息素的化学信号介导的。在大多数蛾类中，雄蛾在很大程度上依赖于雌蛾释放的种特异性性信息素来识别和定位大量同域昆虫中合适的交配伴侣。家蚕利用最简单的可能的信息素系统，其中单个信息素组分（E，Z）-10，12-十六碳二烯醇（bombykol）足以引起完全的性行为。我们以前已经表明，性信息素受体BmOR 1介导的雄蚕触角中的bombykol的特异性检测。然而，目前还不清楚性信息素受体是否是触发启动对潜在配偶的定向行为的最低限度的充分的决定因素。使用转基因家蚕表达的性信息素受体PxOR 1的小菜蛾在BmOR 1表达神经元，我们表明，性信息素受体的选择性决定了化学反应的特异性的性行为在家蚕。表达PxOR 1的Escherichol受体神经元对其特异性配体（Z）-11-十六碳烯醛（Z11-16：Ald）以剂量依赖性方式应答。表达PxOR 1的雄蛾对Z11-16：Ald和小菜蛾雌蛾表现出典型的信息素定向行为和交配尝试。转化的bombykol受体神经元没有影响其在触角叶的投射。这些结果表明，激活bombykol受体神经元本身就足以触发完整的性行为。因此，一个单一的基因定义了在蚕性信息素通信的行为选择性。我们的研究结果表明，一个单一的分子决定因素不仅可以作为一个调制器的行为，但也作为一个复杂的物种特异性的行为序列的全有或全无启动。像许多动物物种一样，飞蛾使用称为性信息素的化学信号与异性的同种个体在繁殖过程中进行交流。通常情况下，雄蛾依靠同种雌蛾释放的性信息素来识别和定位它们的配偶。因此，雄蛾对同种信息素的行为偏好是其成功繁殖的关键因素。性信息素受体蛋白表达在专门的触角嗅觉受体神经元中，据报道在性信息素辨别中起着核心作用。然而，性信息素受体特异性和行为偏好之间的因果关系仍有待证明。我们已经在一种遗传学上易处理的蛾类--蚕蛾（silkmori）--中解决了这个问题，因为这种蛾类具有最简单的可能的信息素系统，其中一种信息素物质--家蚕酚--就能诱导性行为。使用转基因蚕蛾表达的性信息素受体从另一个蛾类，我们发现，只有化学特异性的气味受体在bombykol受体神经元决定的行为偏好在雄蚕蛾。我们的研究结果表明，一个复杂的程序性行为的启动可以依赖于一个单一的信息素受体基因的嗅觉受体神经元的人口中表达的属性。
In insects and other animals, intraspecific communication between individuals of the opposite sex is mediated in part by chemical signals called sex pheromones. In most moth species, male moths rely heavily on species-specific sex pheromones emitted by female moths to identify and orient towards an appropriate mating partner among a large number of sympatric insect species. The silkmoth, Bombyx mori, utilizes the simplest possible pheromone system, in which a single pheromone component, (E, Z)-10,12-hexadecadienol (bombykol), is sufficient to elicit full sexual behavior. We have previously shown that the sex pheromone receptor BmOR1 mediates specific detection of bombykol in the antennae of male silkmoths. However, it is unclear whether the sex pheromone receptor is the minimally sufficient determination factor that triggers initiation of orientation behavior towards a potential mate. Using transgenic silkmoths expressing the sex pheromone receptor PxOR1 of the diamondback moth Plutella xylostella in BmOR1-expressing neurons, we show that the selectivity of the sex pheromone receptor determines the chemical response specificity of sexual behavior in the silkmoth. Bombykol receptor neurons expressing PxOR1 responded to its specific ligand, (Z)-11-hexadecenal (Z11-16:Ald), in a dose-dependent manner. Male moths expressing PxOR1 exhibited typical pheromone orientation behavior and copulation attempts in response to Z11-16:Ald and to females of P. xylostella. Transformation of the bombykol receptor neurons had no effect on their projections in the antennal lobe. These results indicate that activation of bombykol receptor neurons alone is sufficient to trigger full sexual behavior. Thus, a single gene defines behavioral selectivity in sex pheromone communication in the silkmoth. Our findings show that a single molecular determinant can not only function as a modulator of behavior but also as an all-or-nothing initiator of a complex species-specific behavioral sequence. Like many animal species, moths use chemical signals called sex pheromones to communicate with conspecific individuals of the opposite sex in the context of reproduction. Typically, male moths depend on sex pheromones emitted by conspecific females to identify and locate their mates. Therefore, the behavioral preference of male moths to conspecific pheromones is a critical factor for successful reproduction. Sex pheromone receptor proteins expressed in specialized antennal olfactory receptor neurons reportedly play a central role in sex pheromone discrimination. However, the causal relationship between sex pheromone receptor specificity and behavioral preference remains to be proven. We have addressed this question in a genetically tractable moth species, the silkmoth (Bombyx mori), because this species possesses the simplest possible pheromone system in which a single pheromone substance, bombykol, elicits full sexual behavior. Using transgenic silkmoths expressing a sex pheromone receptor from another moth species, we revealed that solely the chemical specificity of the odorant receptors in bombykol receptor neurons determines the behavioral preference in male silkmoths. Our results show that the initiation of a complex programmed sexual behavior can depend on the properties of a single pheromone receptor gene expressed in a population of olfactory receptor neurons.
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影响因子：
3.5
作者：
Kanzaki, R;Soo, K;Wada, S
通讯作者：
Wada, S
影响因子：
16.2
作者：
Dobritsa, AA;van der Goes van Naters, W;Carlson, JR
通讯作者：
Carlson, JR
影响因子：
2.8
作者：
Karpati, Zsolt;Dekker, Teun;Hansson, Bill S.
通讯作者：
Hansson, Bill S.
影响因子：
56.9
作者：
Nakagawa, T;Sakurai, T;Touhara, K
通讯作者：
Touhara, K
DOI：
10.1073/pnas.72.9.3337
发表时间：
1975-01-01
影响因子：
11.1
作者：
KIKUCHI, T
通讯作者：
KIKUCHI, T