Biochemical studies on of ecological function of meiobenthos

为了确定日本西南诸岛红树林河口的纤维素降解过程，在冲绳本岛（Gesashi河和Manko河）、石垣岛（Nagura湾和Miyara河）和西表岛（浦内河、海、日内河、Shira河和Maira河）共9个地点检测了沉积物的纤维素酶活性。酶谱分析用于记录所有沉积物样品和小型底栖生物中的纤维素酶活性。一个27-kDa的纤维素酶活性带的几个动物群体中的小型底栖动物，包括Gammaridea，Turbellaria，介形类，寡毛类，线虫，多毛类。然而，红树林海螺，Terebralia palustris，这是一个广泛分布的大型底栖动物在这些地区的成员，除了冲绳，在29，31，49，和56 kDa的纤维素酶活性。有趣的是，31-kDa纤维素酶活性水平都记录在沉积物和粪便中的T。沼泽本研究的结果表明，各种底栖动物有助于在红树林沉积物中的纤维素降解在日本西南诸岛的河口。

To determine the process of cellulose degradation in mangrove estuaries of the South-Western Islands, Japan, the cellulase activity of sediments was examined at a total of 9 sites on Okinawa Main Island (the River Gesashi and Manko), Ishigaki Island (Nagura Gulf and River Miyara), and Iriomote Island (rivers Urauchi, Mare, Hinai, Shira and Maira). Zymographic analysis was used to record cellulase activity in all sediment samples and in the meiobenthos. A 27-kDa cellulase activity band was expressed by several animal groups examined in the meiobenthos, including Gammaridea, Turbellaria, Ostracoda, Oligochaeta, Nematoda, and Polychaeta. However, the mangrove whelk, Terebralia palustris, which is a widely distributed member of the macrobenthos in these areas except Okinawa Main Island, expressed cellulase activities at 29, 31, 49, and 56 kDa. Interestingly, 31-kDa cellulase activity levels were recorded both in sediments and in the feces of T. palustris. The results of this study indicate that various benthic animals contribute to cellulose degradation in mangrove sediments of estuaries in the Southwestern Japanese Islands.