Biochemical studies on of ecological function of meiobenthos

小型底栖动物生态功能的生化研究

• 批准号: 21380131
• 负责人: TOYOHARA Haruhiko
• 金额: $ 11.15万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21380131/
• 关键词: セルラーゼ; セルロース; メイオベントス; 底泥; 湿地帯; マングローブ; ベントス; 干潟; 河川; 河川(底泥

To determine the process of cellulose degradation in mangrove estuaries of the South-Western Islands, Japan, the cellulase activity of sediments was examined at a total of 9 sites on Okinawa Main Island (the River Gesashi and Manko), Ishigaki Island (Nagura Gulf and River Miyara), and Iriomote Island (rivers Urauchi, Mare, Hinai, Shira and Maira). Zymographic analysis was used to record cellulase activity in all sediment samples and in the meiobenthos. A 27-kDa cellulase activity band was expressed by several animal groups examined in the meiobenthos, including Gammaridea, Turbellaria, Ostracoda, Oligochaeta, Nematoda, and Polychaeta. However, the mangrove whelk, Terebralia palustris, which is a widely distributed member of the macrobenthos in these areas except Okinawa Main Island, expressed cellulase activities at 29, 31, 49, and 56 kDa. Interestingly, 31-kDa cellulase activity levels were recorded both in sediments and in the feces of T. palustris. The results of this study indicate that various benthic animals contribute to cellulose degradation in mangrove sediments of estuaries in the Southwestern Japanese Islands.

为了确定日本西南诸岛红树林河口的纤维素降解过程，在冲绳本岛（Gesashi河和Manko河）、石垣岛（Nagura湾和Miyara河）和西表岛（浦内河、海、日内河、Shira河和Maira河）共9个地点检测了沉积物的纤维素酶活性。酶谱分析用于记录所有沉积物样品和小型底栖生物中的纤维素酶活性。一个27-kDa的纤维素酶活性带的几个动物群体中的小型底栖动物，包括Gammaridea，Turbellaria，介形类，寡毛类，线虫，多毛类。然而，红树林海螺，Terebralia palustris，这是一个广泛分布的大型底栖动物在这些地区的成员，除了冲绳，在29，31，49，和56 kDa的纤维素酶活性。有趣的是，31-kDa纤维素酶活性水平都记录在沉积物和粪便中的T。沼泽本研究的结果表明，各种底栖动物有助于在红树林沉积物中的纤维素降解在日本西南诸岛的河口。

项目成果

Occurrence of cellulase activities in mangrove estuarine mysids and Acetes shrimps

红树林河口糠虾和醋酸虾中纤维素酶活性的发生

• 发表时间: 2012
• 期刊: JIRCUS Working Report
• 作者: T. Niiyama;Y. Hanamura;K. Tanaka and H. Toyohara
• 通讯作者: K. Tanaka and H. Toyohara

干潟底泥バイオリアクター説の検証ーヤマトシジミセルラーゼのcellulose binding domain (CBD)のアンカー機能の検討

滩涂泥生物反应器理论的验证——Yamatoshijimi纤维素酶纤维素结合域(CBD)锚定功能的检验

• 发表时间: 2012
• 作者: 茶谷翔太;阪倉良孝;萩原篤志;桑原 卓哉・冨士 泰期・笠井 亮秀・山下 洋・一見 和彦・岩本 有司・荻野 裕平・中野 光・小路 淳;劉文
• 通讯作者: 劉文

Widespread distribution of cellulase and hemicellulase activities among aquatic invertebrates

• DOI: 10.1007/s12562-011-0361-8
• 发表时间: 2011-05
• 期刊: Fisheries Science
• 影响因子: 1.9
• 作者: Takatoshi Niiyama;H. Toyohara

Biochemical Studies on the Cellulase Activities in Macrobenthos in a Mangrove Estuary

红树林河口大型底栖动物纤维素酶活性的生化研究

• 发表时间: 2010
• 作者: Sakai T;Matsuyama T;Nakayasu C;Haruhiko Toyohara
• 通讯作者: Haruhiko Toyohara

Enzymes Involved in the Degradation of Plant-Derived Carbohydrates in the Wet Lands

参与湿地植物源碳水化合物降解的酶

• 发表时间: 2010
• 作者: Haruhiko Toyohara;Kentaro Sakamoto
• 通讯作者: Kentaro Sakamoto