Construction of human antibody library and establishment of selection method to develop fragment antibody therapeutics

本研究利用T7噬菌体展示系统构建了人源单链抗体和美洲驼VHH抗体的文库，并建立了特异性抗体的筛选方法。特别是，我们成功地从文库中分离出与抗原结合的T7噬菌体克隆，这表明我们的文库系统可用于快速产生特异性抗体。另一方面，由于抗体的折叠问题和不稳定性，选择性富集了非特异性结合位点，这些问题有待进一步研究解决。

In this study, we constructed the libraries of human single-chain Fv antibody and llama VHH antibody using T7 phage display system and established the selection method to isolate the specific phages. Especially, we succeeded in the isolation of the T7 phage clones binding to the antigens from the library, which indicates our library system is useful for a rapid generation of specific antibodies. On the other hand, we found the problematic issues that the non-specific binding phages were enriched by selection process because of folding problems and instability of antibody, which should be solved in the further investigations.