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Expression and functional analyses of G protein-coupled receptor GPR49/LGR5 in human tissues and diseases

G蛋白偶联受体GPR49/LGR5在人体组织和疾病中的表达及功能分析

基本信息

批准号：
21390108
负责人：
SAKAMOTO Michiie
金额：
$ 11.81万
依托单位：
Keio University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2009
资助国家：
日本
起止时间：
2009 至 2011
项目状态：
已结题

项目摘要

GPR49/LGR5 is reportedly expressed in stem cells of the intestinal crypts and hair follicles of mice, and is overexpressed in some types of cancer. We showed that there was significant overexpression of LGR5 in 35 of 50 colorectal cancers, and in all sporadic colonic adenomas, compared with matched normal mucosa. LGR5 expression correlated significantly with lymphatic invasion, vascular invasion, tumor depth, lymph node metastasis, and tumor stage(IIIC vs. IIIB). In addition to cancer cells, crypt base columnar cells of the small intestine and colon were shown by in situ hybridization to express LGR5. This is the first report suggesting the involvement of LGR5, not only in early events but also in late events in colorectal tumorigenesis. We also overexpressed or down-regulated LGR5 expression in HCC cell lines to explore the function of LGR5 in cancer cells. In vivo and in vitro data suggested that aberrant expression of LGR5 regulates epithelial cell phenotype and survival

据报道，GPR 49/LGR 5在小鼠肠隐窝和毛囊的干细胞中表达，并且在某些类型的癌症中过表达。我们发现，与匹配的正常粘膜相比，在50例结直肠癌中的35例和所有散发性结肠腺瘤中，LGR 5显著过表达。LGR 5表达与淋巴管浸润、血管浸润、肿瘤深度、淋巴结转移和肿瘤分期显著相关（IIIC vs. IIIB）。除癌细胞外，原位杂交显示小肠和结肠的隐窝基底柱状细胞表达LGR 5。这是第一份报告表明LGR 5不仅参与结直肠肿瘤发生的早期事件，而且参与晚期事件。我们还在HCC细胞系中过表达或下调LGR 5的表达，以探索LGR 5在癌细胞中的功能。体内和体外数据表明，LGR 5的异常表达调节上皮细胞的表型和存活

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Integtegrative pathologic and molecular approach for HCC diagnosis

HCC 诊断的综合病理学和分子方法

DOI：
发表时间：
2011
期刊：
影响因子：
0
作者：
Miyamura H;Nishizawa H;Ota S;Suzuki M;Inagaki A;Egusa H;Nishiyama s;Kato T;Pryor-Koishi K;Nakanishi I;Fujita T;Imayoshi Y;Markoff A;Yanagihara I;Udagawa Y;Kurahashi H;Michiie Sakamoto
通讯作者：
Michiie Sakamoto

Endothelin-2 is upregulated in basal cell carcinoma under control of Hedgehog signaling pathway.

在 Hedgehog 信号通路的控制下，内皮素 2 在基底细胞癌中上调。