Analysis of lung adenocarcinoma according to molecular biological classification

我们根据我们最初列出的四个主题进行了这项研究。对于侵袭前病变和原位病变的分析，我们可以在新的腺癌分类中建立原位腺癌的定义，作为IASLC病理小组的成员。我们仍在使用SNP阵列分析多个病变，这是我们的第二个主题。对于肿瘤内异质性的第三个主题，我们可以证明不同肿瘤内不同的EGFR突变状态是由基因扩增的不均一性分布和突变等位基因特异性失衡共同引起的，我们将这种现象称为假异质性。对于第四个主题，我们也取得了足够的成果，因为我们参与了建立ALK诊断算法的国际合作研究，并将其确立为日本肺癌协会要求的指南。

We carried out this study according to four themes that we initially laid out. For analysis of preinvasive and in-situ lesions, we could establish definition of adenocarcinoma in-situ in the new adenocarcinoma classification as a member of the IASLC pathology panel. We are still analyzing multiple lesions using SNP array that is of our second theme. To the third theme of intra-tumor heterogeneity, we could show that different EGFR mutation status within individual tumors was caused by combination of heterogeneous distribution of gene amplification and mutation allele specific imbalance, and we called the phenomenon"pseudo-heterogeneity". We have also achieved sufficient results for the fourth theme, because we participated in international collaborative studies to establish ALK diagnostic algorithm and established it as a guidance requested from the Japanese Lung Cancer Association.