Structural and functional analysis of the mechanism for HIV-1 evolution/ adaptation

利用细胞内的病毒适应和体外基因工程，我们成功地产生了亲猴HIV-1(HIV-1mt)，它避开了猕猴细胞中存在的所有已知的抗HIV-1因子(CypA、TRIM5、APOBEC3和Tetherin)的抗病毒作用。通过改进HIV-1Mt和猕猴的感染系统，将有可能从实验上分析HIV-1与宿主之间的战斗。这将导致艾滋病毒-1体内评估系统的完成，否则是不可能的。

Using the viral adaptation in cells and genetic engineering in vitro, we have successfully generated macaque-tropic HIV-1(HIV-1mt) that evades the anti-viral effect of all known anti-HIV-1 factors present in macaque cells(CypA, TRIM5., APOBEC3 and Tetherin). By improving the infection system of HIV-1mt and macaque monkeys, it would be possible to analyze experimentally the battle between HIV-1 and hosts. This would lead to the accomplishment of in vivo evaluation system for HIV-1 otherwise impossible.