Development of novel biomarkers for acute kidney disease by metabolome analysis

通过代谢组分析开发急性肾病的新型生物标志物

• 批准号: 21390263
• 负责人: MATSUO Seichi
• 金额: $ 11.81万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21390263/
• 关键词: メタボローム; バイオマーカー; 急性腎障害; 急性腎障害(AKI); メタボローム解析; ミッドカイン; 虚血腎障害; キャピラリー電気泳動装置+質量分析器; 尿中バイオマーカー

We conducted the metabolome analyses of the urine samples from various renal diseases, and succeeded in obtaining the fundamentally metabolome profile for every renal disease. By comparing each metabolite in AKI group with that in non-AKI group, we were able to acquire a group of biomarker candidates which showed specific concentrations in AKI patients. Using series of urine samples taken from patients who underwent abdominal-aortic-aneurysm replacement surgery before, during and after the operation, until the time of leaving from postoperative ICU, I succeeded in getting urinary metabolome profile of AKI group and non-AKI group. Moreover, compared the time-series variation of each metabolite concentration, I was able to acquire the novel urinary biomarker candidate metabolites group which make it possible to detect AKI early and to extrapolate the prognosis of renal function before and/or during the operation. Information on these various biomarker candidate metabolites groups gives us not only the target substances for the AKI diagnostics but also a key to understanding the mechanisms of AKI.

我们对各种肾脏疾病的尿样进行了代谢组学分析，并成功地获得了每种肾脏疾病的基本代谢组学谱。通过比较阿基组和非AKI组中的每种代谢物，我们能够获得一组在阿基患者中显示特定浓度的生物标志物候选物。使用从接受腹主动脉瘤置换术的患者术前、术中和术后直到离开术后ICU时采集的一系列尿液样本，我成功地获得了阿基组和非AKI组的尿液代谢物组谱。此外，通过比较每种代谢物浓度的时间序列变化，我能够获得新的尿液生物标志物候选代谢物组，这使得有可能早期检测阿基并推断手术前和/或手术期间肾功能的预后。关于这些不同的生物标志物候选代谢物组的信息不仅为我们提供了阿基诊断的靶物质，而且也是理解阿基机制的关键。

项目成果

厚労省難治性疾患克服研究事業:進行性腎障害に関する調査研究

厚生劳动省攻克疑难杂症研究事业：进行性肾脏疾病的研究

• 发表时间: 2011
• 作者: Ito M;Suzuki Y;Okada T;FukudomeT;Yoshimura T;Masuda A;Takeda S;Krejci E;Ohno K;丸山彰一
• 通讯作者: 丸山彰一

Prognostic value of reduced left ventricular ejection fraction at start of hemodialysis therapy on cardiovascular and all-cause mortality in end-stage renal disease patients.

• DOI: 10.2215/cjn.00050110
• 发表时间: 2010-10
• 期刊: Clinical journal of the American Society of Nephrology : CJASN
• 作者: S. Yamada;H. Ishii;Hiroshi Takahashi;T. Aoyama;Yasuhiro Morita;Hirotake Kasuga;Keiko Kimura;Yutaka Ito;R. Takahashi;T. Toriyama;Y. Yasuda;M. Hayashi;H. Kamiya;Y. Yuzawa;S. Maruyama;S. Matsuo;T. Matsubara;T. Murohara

Chronic Kidney Disease Japan Cohort study: baseline characteristics and factors associated with causative diseases and renal function

• DOI: 10.1007/s10157-010-0328-6
• 发表时间: 2010-12-01
• 期刊: CLINICAL AND EXPERIMENTAL NEPHROLOGY
• 影响因子: 2.3
• 作者: Imai, Enyu;Matsuo, Seiichi;Hishida, Akira
• 通讯作者: Hishida, Akira

A Comparison of the Acute Kidney Injury Network and Kidney Disease : Improving Global Outcomes Criteria for Acute Kidney Injury in Critically Ill Patients

急性肾损伤网络与肾脏疾病的比较：改善危重患者急性肾损伤的全球结局标准

• 发表时间: 2011
• 作者: Tanaka R;Sasaki-Ikesawa K;Shimura H;Nishioka K;Hattori N;Tanaka S;Kaji R.;Shinjo H
• 通讯作者: Shinjo H

Reduced number of circulating CD34-positivecells is associated with poor cardiovascular outcomes in chronic hemodialysis patients

循环 CD34 阳性细胞数量减少与慢性血液透析患者心血管结局不佳相关

• 发表时间: 2010
• 作者: Miyazaki K;Ohta Y;Morimoto N;Kurata T;Takehisa Y;Ikeda Y;Matsuura T;Abe K;Shoichi Maruyama
• 通讯作者: Shoichi Maruyama