Establishment of monitoring system with AID-associated microRNA-155 for cutaneous T-cell lymphoma and its application for therapy

AID相关microRNA-155皮肤T细胞淋巴瘤监测系统的建立及其治疗应用

• 批准号: 21390328
• 负责人: TOKURA Yoshiki
• 金额: $ 9.32万
• 依托单位: Hamamatsu University School of Medicine (2010-2011)University of Occupational and Environmental Health, Japan (2009)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21390328/
• 关键词: 皮膚T細胞リンパ腫; AID; 成人T細胞白血病/リンパ腫; マイクロRNA; 菌状息肉症; セザリー症候群; リンパ腫; 皮疹; HTLV-1; 成人T細胞白血病

It has been reported that AID is involved in the development of virus-associated neoplasms. Moreover, microRNA-155 downregulates the expression of AID. On the other hand, point mutations of p53 have been reported in the patients with cutaneous T-cell lymphoma(CTCL) including ATLL. By real-time PCR analysis, we investigated the expression of AID and microRNA-155 in the peripheral blood of patients with mycosis fungoides, Sezary syndrome, and ATLL in a comparison with normal healthy subjects. We found that AID expression was higher in the peripheral blood lymphocytes and tumor-infiltrating cells in CTCL, especially ATLL. We isolated circulating CD4-, CD4+CD25-, and CD4+CD25+cells by MACS and examined the mRNA expression of AID and microRNA-155 in each fraction. In addition, skin specimens and lymph nodes from the patients were also tested. We found that AID expression was elevated with decreased expression of microRNA-155, particularly in ATLL, suggesting that AID and its regulatory microRNA are involved in the development of this virus-associated lymphoma.

有报道称，AID参与了病毒相关肿瘤的发生。此外，microRNA-155还下调了AID的表达。另一方面，已有报道在包括ATLL在内的皮肤T细胞淋巴瘤(CTCL)患者中发现P53点突变。采用实时荧光定量聚合酶链式反应(RT-PCR)技术，检测了真菌样肉芽肿、Sezary综合征和ATLL患者外周血中AID和microRNA-155的表达，并与正常对照组进行了比较。我们发现AID在CTCL的外周血淋巴细胞和肿瘤浸润性细胞中有较高的表达，尤其是ATLL。我们通过MACS分离了循环中的CD4-、CD4+CD25-和CD4+CD25+细胞，并检测了每一组分中AID和microRNA-155的mRNA表达。此外，还对患者的皮肤标本和淋巴结进行了检测。我们发现AID的表达增加，而microRNA-155的表达降低，特别是在ATLL中，提示AID及其调节的microRNA参与了这种病毒相关淋巴瘤的发展。

项目成果

Circulating Th17 cell fluctuation in psoriatic patients treated with topical calcipotriol and betamethasone butyrate propionate

局部用卡泊三醇和丁酸丙酸倍他米松治疗银屑病患者的循环 Th17 细胞波动

• DOI: 10.1111/j.1468-3083.2010.03714.x
• 发表时间: 2011
• 期刊: J Eur Acad Dermatol Venereol
• 作者: Hino R;Kabashima R;Kawakami C;Sugita K;Nakamura M;Tokura Y
• 通讯作者: Tokura Y

新しい抗ヒスタミン薬とその特徴

新型抗组胺药及其特点

• 发表时间: 2011
• 作者: Onishi H;Katano M;戸倉新樹
• 通讯作者: 戸倉新樹

外因性・内因性という分別はアトピー性皮膚炎に必要か

是否有必要区分特应性皮炎的外因和内因？

• 发表时间: 2011
• 作者: 吉村耕一;青木浩樹;小櫃由樹生;重松宏;松崎益徳;Sawai S;戸倉新樹
• 通讯作者: 戸倉新樹

CD8+ CD56+mycosis fungoides with an indolent clinical behavior : case report and literature review.

CD8 CD56 具有惰性临床行为的蕈样肉芽肿：病例报告和文献综述。

• 发表时间: 2010
• 期刊: Acta Derm Venereol (In press)
• 作者: Matsui H;Hamuro M;Nakamura K;Kayahara H;Murano K;Kotsuka Y;Miki Y;Sawada Y
• 通讯作者: Sawada Y

Food-dependent anaphylaxis with serum IgE immunoreactive to dairy products containing high-molecular-weight proteins

食物依赖性过敏反应，血清 IgE 对含有高分子量蛋白质的乳制品产生免疫反应

• 发表时间: 2010
• 期刊: J Dermatol Sci
• 影响因子: 4.6
• 作者: Abe S;Kabashima K;Moriyama T;Tokura Y
• 通讯作者: Tokura Y