Establishment of monitoring system with AID-associated microRNA-155 for cutaneous T-cell lymphoma and its application for therapy

点击翻译按钮获取中文摘要

It has been reported that AID is involved in the development of virus-associated neoplasms. Moreover, microRNA-155 downregulates the expression of AID. On the other hand, point mutations of p53 have been reported in the patients with cutaneous T-cell lymphoma(CTCL) including ATLL. By real-time PCR analysis, we investigated the expression of AID and microRNA-155 in the peripheral blood of patients with mycosis fungoides, Sezary syndrome, and ATLL in a comparison with normal healthy subjects. We found that AID expression was higher in the peripheral blood lymphocytes and tumor-infiltrating cells in CTCL, especially ATLL. We isolated circulating CD4-, CD4+CD25-, and CD4+CD25+cells by MACS and examined the mRNA expression of AID and microRNA-155 in each fraction. In addition, skin specimens and lymph nodes from the patients were also tested. We found that AID expression was elevated with decreased expression of microRNA-155, particularly in ATLL, suggesting that AID and its regulatory microRNA are involved in the development of this virus-associated lymphoma.