Establishment of monitoring system with AID-associated microRNA-155 for cutaneous T-cell lymphoma and its application for therapy

有报道称，AID参与了病毒相关肿瘤的发生。此外，microRNA-155还下调了AID的表达。另一方面，已有报道在包括ATLL在内的皮肤T细胞淋巴瘤(CTCL)患者中发现P53点突变。采用实时荧光定量聚合酶链式反应(RT-PCR)技术，检测了真菌样肉芽肿、Sezary综合征和ATLL患者外周血中AID和microRNA-155的表达，并与正常对照组进行了比较。我们发现AID在CTCL的外周血淋巴细胞和肿瘤浸润性细胞中有较高的表达，尤其是ATLL。我们通过MACS分离了循环中的CD4-、CD4+CD25-和CD4+CD25+细胞，并检测了每一组分中AID和microRNA-155的mRNA表达。此外，还对患者的皮肤标本和淋巴结进行了检测。我们发现AID的表达增加，而microRNA-155的表达降低，特别是在ATLL中，提示AID及其调节的microRNA参与了这种病毒相关淋巴瘤的发展。

It has been reported that AID is involved in the development of virus-associated neoplasms. Moreover, microRNA-155 downregulates the expression of AID. On the other hand, point mutations of p53 have been reported in the patients with cutaneous T-cell lymphoma(CTCL) including ATLL. By real-time PCR analysis, we investigated the expression of AID and microRNA-155 in the peripheral blood of patients with mycosis fungoides, Sezary syndrome, and ATLL in a comparison with normal healthy subjects. We found that AID expression was higher in the peripheral blood lymphocytes and tumor-infiltrating cells in CTCL, especially ATLL. We isolated circulating CD4-, CD4+CD25-, and CD4+CD25+cells by MACS and examined the mRNA expression of AID and microRNA-155 in each fraction. In addition, skin specimens and lymph nodes from the patients were also tested. We found that AID expression was elevated with decreased expression of microRNA-155, particularly in ATLL, suggesting that AID and its regulatory microRNA are involved in the development of this virus-associated lymphoma.