Development of New Strategy of Immunological Tolerance Inductionby Intraportal administration of donor specific Mesenchymal hepatic stem cell in OrganTransplantation

器官移植中供体特异性间充质肝干细胞门静脉注射诱导免疫耐受新策略的开发

• 批准号: 21390356
• 负责人: SATO Yoshinobu
• 金额: $ 11.15万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21390356/
• 关键词: 移植・再生医療; 免疫学; 外科; 免疫寛容; ドナー特異的抗原; 肝幹細胞; 移植; 臓器移植; 拒絶反応; ドナー特異的抗原門脈内投与; 調節性T細胞; shear stress

The purpose of this study was to analyze the mechanisms of theinduction of operational tolerance in clinical liver transplantation by the intra-portaladministration of donor specific antigen and make the new method of induction of toleranceby hepatic stem cell. In the basic examination, the CD133+ NCAM+ human hepaticstem/progenitor cell was recognized around the region with existence of ductular reactionin the human liver with chronic or acute hepatitis. Especially, its existence was moreclearly in the human liver with higher hepatic injury. However, it was not recognizedin the normal human liver. In the immunological analysis of intra-portal administrationof super donor antigens, the population of FoxP3^+/CD4^+CD25^+ regulatory T cells increasedin the transplant patients with no immunosuppressants.Treg. cells increased in the patients above MELD score 20. It was proved that the graspof preoperative immunological situation of transplant patients take the betteradministration of immunosuppressants.

这项研究的目的是分析通过供体特异性抗原的体内分泌的临床肝移植​​中操作耐受性的机制，并使诱导耐受性肝干细胞的新方法。在基本检查中，CD133+ NCAM+人类肝系统/祖细胞在该区域被识别，并存在慢性或急性肝炎的人肝脏中的导管反应。尤其是，它的存在在人类肝脏中以较高的肝损伤状态被病。但是，它并不认识到正常的人类肝脏。在超级供体抗原的体内给药的免疫学分析中，Foxp3^+/CD4^+CD25^+调节性T细胞的种群增加了没有免疫抑制剂的移植患者。以上MELD评分20的患者的细胞增加了20。

项目成果

Auxuliary partial orthotopic living donor liver transplantation for fulminant hepatic failure with flat electroencephalogram : A case of report

辅助部分原位活体肝移植治疗暴发性肝衰竭伴平脑电图一例报告

• 发表时间: 2010
• 期刊: Transplantation Proceedings
• 影响因子: 0.9
• 作者: Kobayashi T;et al
• 通讯作者: et al

Impact of FoxP3+CD4+CD25+T Cells in the Recipients Acquiring Almost Tolerance

FoxP3 CD4 CD25 T 细胞对获得几乎耐受的受体的影响

• 发表时间: 2009
• 作者: Kai M;Onishi H;Katano M;et al;Yoshinobu Sato
• 通讯作者: Yoshinobu Sato

Intra portal donorspecific antigen transfusion mightprevent re-infection of hepatitis Cvirus after living related livertransplantation in hepatitis C hepaticcirrhosis

门静脉内供体特异性抗原输注可预防丙型肝炎肝硬化活体肝移植后丙型肝炎病毒的再感染

• 发表时间: 2009
• 期刊: Hepatogastroenterology
• 作者: Sato Y;Oya H;Yamamoto S;Kobayashi T,Watanabe T;Kokai H;Yamagiwa S,Hatakeyama K
• 通讯作者: Yamagiwa S,Hatakeyama K

Intra portal donor specific antigen transfusion might prevent re-infecti…

门静脉内供者特异性抗原输注可能会预防再次感染……

• 发表时间: 2009
• 期刊: Hepato-Gastroenterology 56
• 作者: Y Sato;H Oya;et al
• 通讯作者: et al

Induction of operational tolerance and its mechanism by intraportal administration of donor specific antigens in living-donor liver transplantation

活体肝移植中供者特异性抗原门静脉注射诱导操作耐受及其机制

• 发表时间: 2010
• 作者: Sato Y;Yamamoto S;Oya H;et al
• 通讯作者: et al