Detection of Circulating Endothelial Cells in Severe Sepsis

严重脓毒症中循环内皮细胞的检测

• 批准号: 21390482
• 负责人: KOIKE Kaoru
• 金额: $ 11.65万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21390482/
• 关键词: 敗血症; 遊離型血管内皮細胞; CEC; AP-1; TAK-1; NF-κB; 播種性血管愛凝固症候群; DIC; 血管内皮細胞; アポトーシス; ショック; 遺伝子核酸治療; マウス; Death受容体

Sepsis is a severe disorder as systemic inflammation with infection. In this illness, vascular endothelial damage proceeds to induce disseminated intravascular coagulation(DIC). This study revealed that circulating endothelial cell(CEC) was increased and plasma platelet count was decreased in the time dependent manner after sepsis induction by cecal ligation and puncture in male BALB-C mice. Nuclear factor-kappaB decoy oligonucleotides more strongly decreased CEC count than inactivation of FADD, TAK-1, and activator protein-1. The CEC count could be a reliable marker to evaluate DIC and dysfunction of vascular endothelium.

脓毒症是一种严重的全身性炎症和感染性疾病。在这种疾病中，血管内皮损伤导致弥散性血管内凝血（DIC）。本研究表明，雄性BALB-C小鼠盲肠结扎穿孔诱导脓毒症后，循环内皮细胞（CEC）增加，血浆血小板计数下降，呈时间依赖性。核因子-kappaB诱饵寡核苷酸更强烈地减少CEC计数比FADD，TAK-1和激活蛋白-1的失活。CEC计数可作为评价DIC及血管内皮功能障碍的可靠指标。

项目成果

Up-regulation of histamine H4receptors contributes to splenicapoptosis in septic mice

组胺 H4 受体上调导致脓毒症小鼠脾细胞凋亡

• 发表时间: 2010
• 期刊: J Pharmacol Exp Ther
• 影响因子: 3.5
• 作者: Matsuda N;Teramae H;Futatsugi M;Takano K;Yamamoto S;Tomita K;SuzukiT;Yokoo H;Koike K;Hattori Y
• 通讯作者: Hattori Y