TO ELUCIDATE THE MECHANISM OF REMOTE ORGAN DYSFUNCTION AFTER INTESTINAL ISCHEMIA-REPERFUSION

阐明肠缺血再灌注后远端器官功能障碍的机制

• 批准号: 14571416
• 负责人: KOIKE Kaoru
• 金额: $ 2.18万
• 依托单位: TOHOKU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571416/
• 关键词: intestine; ischemia; reperfusion injury; brain injury; hemorrhagic shock; Magnetic Resonance Spectroscopy; metabolism; Magnetic Resonance Imaging; リンパ液; 脂質メデイエーター; 磁気共鳴装置; 脳; 脳波

1.Magnetic resonance spectroscopy (MRS) technique provides useful information for the understanding of tissue metabolic changes. We used this technique to investigate the effect of gut ischemia-reperfusion (IR) on the brain. [In vivo 31P MRS] All rats survived more than seven days after gut IR (45min of superior mesenteric artery occlusion). The IR group showed a significant elevation of both phosphomonoesters (PME) and phosphodiesters (PDE) signals on day 2. The PME signal represents phosphocholine, phosphoethanolamine, etc., and the PDE signal indicate membrane degeneration. Compared to normal control animals, lower pH values were observed in both groups. Operation and repeated anesthesia may have produced acidic metabolites such as lactate. [In vitro 1H MRS] The IR group indicated significantly higer levels of N-acetylaspartate, creatines, myo-inositol, lactate and alanine than the sham group on the day 5. These data suggest gut IR provokes metabolic changes in the brain.2.Biologica … More l activities of mesenteric lymph after hemorrhagic shock on neutrophils : Previous studies have demonstrated that the ligation of mesenteric lymph duct prevents hernorrhagic shock-induced lung injury. We hypothesized that mesenteric lymph following hemorrhagic shock activates neutrophils (PMNs). Rats were exposed to hemorrhagic shock (40mmHg, 30min) and resuscitated over 2 hours with shed blood + normal saline (shed blood x 2) (HSgroup). Asham group underwent identical procedures without hemorrhage and resuscitation. We collected mesenteric lymph between 1 and 2 hours during the resuscitation phase. Superoxide anion production was measured with the superoxide dismutase inhibitable cytochrome C reduction method. PMS adhesion was measured by the radioactivity of adherent PMNs labeled with 51Cr to fibrinogen, and CD11b surface expression was determined by flow cytometry. Mesenteric lymph in the HS group significantly increased superoxide production, PMN adherence and CDT lb surface expression compared to the sham group. Mesenteric lymph may play a crucial role in the pathogenesis of multiple organ dysfunction syndrome. Less

1.磁共振波谱（MRS）技术为了解组织代谢变化提供了有用的信息。我们用这种技术来研究肠缺血再灌注（IR）对脑的影响。[In在肠缺血再灌注（上级肠系膜动脉阻断45分钟）后，所有大鼠均存活超过7天。IR组在第2天表现出磷酸单酯（PME）和磷酸二酯（PDE）信号的显著升高。PME信号代表磷酸胆碱、磷酸乙醇胺等，PDE信号表明膜变性。与正常对照动物相比，两组中均观察到较低的pH值。手术和反复麻醉可能会产生酸性代谢物，如乳酸盐。[In IR组在第5天的N-乙酰天冬氨酸、肌酸、肌醇、乳酸和丙氨酸水平显著高于假手术组。这些数据表明肠道IR引起大脑的代谢变化。2.生物学 ...更多信息 失血性休克后肠系膜淋巴管对中性粒细胞的影响：以往的研究表明，结扎肠系膜淋巴管可防止失血性休克引起的肺损伤。我们假设失血性休克后肠系膜淋巴液激活中性粒细胞。失血性休克（40 mmHg，30 min）后用放血+生理盐水（放血× 2）复苏2 h以上（HS组）。Asham组手术方法相同，无出血和复苏。我们在复苏阶段的1至2小时之间收集肠系膜淋巴液。超氧化物歧化酶可降解细胞色素C还原法测定超氧阴离子的产生。PMS粘附通过51 Cr标记的粘附的PMNs对纤维蛋白原的放射性来测量，并且通过流式细胞术来确定CD 11b表面表达。与假手术组相比，HS组的肠系膜淋巴液显著增加超氧化物产生、PMN粘附和CDT Ib表面表达。肠系膜淋巴结可能在多器官功能障碍综合征的发病机制中起重要作用。少