Elucidation of calcified tissue regeneration mechanism using dental pulp cells

利用牙髓细胞阐明钙化组织再生机制

• 批准号: 21390550
• 负责人: MIYAZAWA Hiroo
• 金额: $ 11.56万
• 依托单位: Matsumoto Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21390550/
• 关键词: 歯髄細胞; 歯根膜細胞; 石灰化; 骨形成; アルカリホスファターゼ; アリザリンレッド染色; ウシ胎児血清; 無血清培地; 再生医学; 石灰化機構; 硬組織再生

Dental pulp cells are considered to be a promising tool for the regeneration of hard tissues, due to their multipotency. The goal of the present study is to establish a simple method of regenerating hard tissue using dental pulp cells. Toward this end, we characterized dental pulp cells in comparison with osteoblasts and explored the mechanism of calcification. Mouse dental pulp cells exhibited strong alkaline phosphatase and extracellular matrix calcification activity even in the absence of exogenous rhBMP-2.A Genechip analysis showed the overall gene expression in dental pulp cells to be similar to that in osteoblasts, though dental pulp cells expressed higher levels of BMPs, annexin A8(a Ca channel), and solute carrier protein 20a2(Slc20a2)(a Pi transporter) than osteoblasts. The transplanted dental pulp cells into immunodeficiency mice exert bone regenerative activity in vivo.

由于其多能量，牙髓细胞被认为是硬组织再生的有前途的工具。本研究的目的是建立一种使用牙髓细胞再生硬组织的简单方法。为此，我们与成骨细胞相比，表征了牙髓细胞，并探讨了钙化的机理。 Mouse dental pulp cells exhibited strong alkaline phosphatase and extracellular matrix calcification activity even in the absence of exogenous rhBMP-2.A Genechip analysis showed the overall gene expression in dental pulp cells to be similar to that in osteoblasts, though dental pulp cells expressed higher levels of BMPs, annexin A8(a Ca channel), and solute carrier protein 20A2（SLC20A2）（PI转运蛋白）比成骨细胞。移植的牙髓细胞中的免疫缺陷小鼠在体内发挥骨再生活性。

项目成果

骨粗鬆症とRANKLシグナル

骨质疏松症和 RANKL 信号

• 发表时间: 2011
• 期刊: CLINICAL CALCIUM
• 作者: 中村美どり;宇田川信之
• 通讯作者: 宇田川信之

破骨細胞形成におけるIL-34の役割

IL-34 在破骨细胞生成中的作用

• 发表时间: 2010
• 作者: Okuda K;Hanada N;Usui Y;Takeuchi H;Koba H;Nakao R;Watanabe H;and Senpuku H;中道裕子
• 通讯作者: 中道裕子

Regulation of osteoclast function. Identification of cell cycle-arrested quiescent osteoclast precursors in vivo

破骨细胞功能的调节。

• 发表时间: 2011
• 期刊: Modern Rheumatology
• 影响因子: 2.2
• 作者: Nakamura I;Udagawa N(他3名)
• 通讯作者: Udagawa N(他3名)

Function of OPG as a Traffic Regulator for RANKL Is Crucial for Controlled Osteoclastogenesis

• DOI: 10.1002/jbmr.89
• 发表时间: 2010-09-01
• 期刊: JOURNAL OF BONE AND MINERAL RESEARCH
• 影响因子: 6.2
• 作者: Aoki, Shigeki;Honma, Masashi;Suzuki, Hiroshi
• 通讯作者: Suzuki, Hiroshi

破骨細胞前駆細胞の形成と供給におけるM-CSFとIL-34の役割

M-CSF和IL-34在破骨细胞前体细胞形成和供应中的作用

• 发表时间: 2010
• 作者: Yoshimi;R;中道裕子
• 通讯作者: 中道裕子