Statistical Pattern Recognition in Small Sample Size Situations and its Application to Detection of Hepatocellular Carcinoma

小样本情况下的统计模式识别及其在肝细胞癌检测中的应用

• 批准号: 21500173
• 负责人: HAMAMOTO Yoshihiko
• 金额: $ 2.91万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21500173/
• 关键词: 癌; パターン認識; 診断

The purpose of this study is to develop a novel blood test system for detecting hepatocellular carcinoma(HCC) associated with hepatitics C virus(HCV) infection. In order to develop the novel test system, we must establish a feature selection method to find effective bio-makers in small training sample size situations, compared with very high dimensions. We found 2 methylated genes(SPINT2 and SRD5A2). With use of 2 methylated genes, AFP. and PIVKA II. we designed the blood test system for detecting HCC, which achieved high abilities(73. 2% sensitivity and 87. 7% specificity) for 258 test samples provided from 4 institutes. This suggests that our blood test system might lead to clinical applications.

本研究的目的是建立一种新的血液检测系统，用于检测与丙型肝炎病毒（HCV）感染相关的肝细胞癌（HCC）。为了开发新的测试系统，我们必须建立一个特征选择方法，以找到有效的生物标记在小训练样本量的情况下，相比非常高的维度。我们发现了2个甲基化基因（SPINT 2和SRD 5A2）。使用2个甲基化基因，AFP。PIVKA II。我们设计了一套检测肝癌的血液检测系统，该系统具有较高的检测能力（73，2%的灵敏度和87。7%特异性）。这表明我们的血液检测系统可能会导致临床应用。

项目成果

Rapid and quantitative detection of CpG-methylation status using TaqMan PCR combined with methyl-binding-domain polypeptide

• DOI: 10.1016/j.clinbiochem.2009.03.017
• 发表时间: 2009-07-01
• 期刊: CLINICAL BIOCHEMISTRY
• 影响因子: 2.8
• 作者: Kimura, Naoki;Moribe, Toyoki;Oka, Masaaki
• 通讯作者: Oka, Masaaki

An optimized Fisher linear classifier for detection of hepatocellular carcinoma, 8^<th> AACR-JCA Joint Conference Cancer Genomics

用于检测肝细胞癌的优化 Fisher 线性分类器，第 8 届 AACR-JCA 癌症基因组学联合会议

• 发表时间: 2010
• 作者: Y. Fujita;Y. Hamamoto;N. Iizuka;M. Oka;T. Moribe
• 通讯作者: T. Moribe

特許

专利

• 发表时间: 2008

統計的パターン認識入門

统计模式识别简介

• 发表时间: 2009
• 作者: Y. Fujita;Y. Hamamoto;N. Iizuka;M. Oka;T. Moribe;Y.Fujita;浜本義彦
• 通讯作者: 浜本義彦

Methylated cyclin D2 gene circulating in the blood as a prognosis predictor of hepatocellular carcinoma.

• DOI: 10.1016/j.cca.2010.01.004
• 发表时间: 2010-04
• 期刊: Clinica chimica acta; international journal of clinical chemistry
• 作者: M. Tsutsui;N. Iizuka;T. Moribe;T. Miura;Naoki Kimura;S. Tamatsukuri;H. Ishitsuka;Y. Fujita;Y. H