Development of a red fluorescent Ca^<2+> sensor

本研究的目的是开发一种基于荧光蛋白的红色荧光Ca^<2+>传感器，该传感器可以被红光激发并发出红色荧光。我们用循环排列的RFP取代G-CaMP2中原来的绿色荧光Ca^<2+>传感器的循环排列GFP结构域，生成了红色荧光Ca^<2+>传感器原型，然后对该原型传感器进行随机诱变，筛选出高性能的变体。结果，我们成功地发现了称为R-CaMP的传感器，它在Ca^<2+>结合后显示出很大的红色荧光变化。

The purpose of this study is to develop a fluorescent protein-based red fluorescent Ca^<2+> sensor which can be excited by red light and emit red fluorescence. We generated a prototype red fluorescent Ca^<2+> sensor by replacing the circularly permuted GFP domain of G-CaMP2, which is our original green fluorescent Ca^<2+> sensor, with the circularly permuted RFP, then randomly mutagenized this prototype sensor and screened variants with high performance. As a result, we successfully found the sensor termed R-CaMP, which showed a great red fluorescence change upon Ca^<2+> binding.