EXCITATION-CONTRUCTION COUPLING AND TETRAD STRUCTURE OF L-TYPE CACLIUM CHANNEL.

L型钙通道的激励-构造耦合和四分体结构。

• 批准号: 11680813
• 负责人: NAKAI Junichi
• 金额: $ 2.3万
• 依托单位: OKAZAKI NATIONAL RESEARCH INSTITUTES
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11680813/
• 关键词: EXCITATION-CONTRUCTION COUPLING; RYANODINE RECEPTOR; CALCIUM CHANNEL; カルシウム放出チャネル

Voltage-gated L-type calcium channels (Dihydropyridine receptors) and ryanodine receptors (calcium release channels) play important roles in muscle contruction. In this research, chimeras of the skeletal muscle (RyR1) and cardiac muscle (RyR2) ryanodine receptor were expressed in HEK293 cells to assess their calcium dependence in [^3H] ryanodine binding and single channel measurements. The results indicate that the C-terminal one-fourth has a major role in calcium activation and inactivation of RyR1. Further, our results show that replacement of RyR1 regions corresponding RyR2 regions can result in loss and/or reduction of [^3H] ryanodine binding affinity while maintaining channel activity.

电压门控L型钙通道（二氢吡啶受体）和兰尼碱受体（钙释放通道）在肌肉收缩中起重要作用。在这项研究中，骨骼肌（RyR 1）和心肌（RyR 2）兰尼碱受体的嵌合体在HEK 293细胞中表达，以评估它们在[^3H]兰尼碱结合和单通道测量中的钙依赖性。结果表明，C-末端四分之一在钙激活和RyR 1失活中起主要作用。此外，我们的研究结果表明，RyR 1区域对应RyR 2区域的替换可以导致[^3H]兰尼碱结合亲和力的丧失和/或降低，同时保持通道活性。