Study of specifically patterned localization of proteins based on Biobased polymer

在这项研究中，我尝试使用生物基聚合物的自图案化技术来图案化和定位生物基聚合物基质上的蛋白质。我们成功地获得了独特的图案化凝胶的基础上的自图案化的生物基聚合物，通过改变参数，如制备温度和聚合物的浓度。通过使用两种不同的多糖的半乳糖化，也可以获得分别固定葡萄糖氧化酶和过氧化氢酶的JAUNS颗粒，所述两种不同的多糖可以通过不同的盐形成凝胶。VCH霍乱毒素是一种膜蛋白，它能诱导DMPC和胆固醇组成的单层膜发生相分离，使VCH定位于胆固醇富集相。

In this study, I attempted to use the technique of self-patterning of biobased polymer to pattern and localize proteins on the biobased polymer matrix. We successfully obtained uniquely patterned gels based on the self-patterning of biobased polymer by changing parameter such as preparation temperature and concentration of the polymer. JAUNS particles immobilizing glucose oxidase and catalase separately can be also obtained by using the galation of two different polysaccharides that can form gels by different salts. It was also found that VCH cholera toxin, a membrane protein, induces phase separation of monolayer composed of DMPC and Cholesterol, which lead to localize VCH in the Cholesterol rich phase.