Study of specifically patterned localization of proteins based on Biobased polymer

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In this study, I attempted to use the technique of self-patterning of biobased polymer to pattern and localize proteins on the biobased polymer matrix. We successfully obtained uniquely patterned gels based on the self-patterning of biobased polymer by changing parameter such as preparation temperature and concentration of the polymer. JAUNS particles immobilizing glucose oxidase and catalase separately can be also obtained by using the galation of two different polysaccharides that can form gels by different salts. It was also found that VCH cholera toxin, a membrane protein, induces phase separation of monolayer composed of DMPC and Cholesterol, which lead to localize VCH in the Cholesterol rich phase.