Evaluation of adhesivity of blood cells on solid surfaces using the inclined centrifuge microscope

中性粒细胞是最丰富的白细胞类型。已知它们粘附在小静脉的内皮细胞上，并且比全血更慢地沿血管沿着。在内径小于中性粒细胞的细小毛细血管中，细胞可以堵塞血管以停止血液流动。因此，了解微血管中的血流特性或中性粒细胞在免疫反应中的行为，阐明血细胞与固体表面之间的相互作用是至关重要的。在本研究中，我们观察了HL 60细胞，一种中性粒细胞的模型细胞，在平板玻璃板或培养的内皮细胞使用倾斜离心显微镜的运动，以定量评估这些固体表面上的血细胞的粘附特性。

Neutrophils are the most abundant type of leukocytes. It is known that they adhere onto endothelial cells of venules and roll along the vessels slower than the whole blood. In fine capillaries whose inner diameter is smaller than the neutrophils, the cells could plug the vessels to cease blood flow. Therefore, it is essential to understand blood flow characteristics in microvasculature or behavior of neutrophils in immune response that clarifying interaction between the blood cells and the solid surfaces. In this research, we observed motion of HL60 cells, a kind of model cell of neutrophils, on a flat glass plate or cultured endothelial cells using the inclined centrifuge microscope to quantitatively evaluate adhesive characteristics of the blood cells on those solid surfaces.