Experimental observation of surface morphology of endothelium on motion of neutrophils using inclined centrifuge microscope

用倾斜离心显微镜观察在平板玻璃板培养的HUVECs上，中性粒细胞样HL60细胞在压力作用下的运动。观察到细胞沿凸型HUVECs边缘区域移动。这表明内皮细胞的表面形态增加了中性粒细胞与内皮细胞粘附的可能性，因为中性粒细胞滚动所必需的p -选择素在内皮细胞的边缘区域广泛表达。在受剪切应力作用的定向HUVECs上，HL60细胞的运动比在无定向HUVECs上更直线，但其平均运动速度不随方向的变化而变化。由于未分化的HL60细胞在定向HUVECs上的平均速度增加，因此分化后的HL60细胞对HUVECs的粘附性被认为是调节平均速度的主要因素。

Motion of neutrophil-like HL60 cells under a pressing fore on HUVECs cultured on a flat glass plate was observed using the inclined centrifuge microscope. It was observed that the cells move along marginal regions of convex HUVECs. This suggests that the surface morphology of endothelium enhances possibility of adhesion between neutrophils and the endothelium because P-selectin which is necessary for neutrophils' rolling is extensively expressed on the marginal region of the endothelial cells. The HL60 cells move more straight on oriented HUVECs which had been exposed to a shear stress than on not-oriented HUVECs, but their mean velocity was not changed with the orientation. Since the mean velocity of undifferentiated HL60 cells increased on the oriented HUVECs, adhesivity of the differentiated HL60 cells to the HUVECs is considered to be a dominant factor to regulate the mean velocity.