Eiucidation of the mobilization mechanism of transposab leel ements

长散布元件（LINES）是在真核生物基因组中移动和扩增其自身拷贝的转座元件。LINE通过一种称为逆转录转座的机制移动，其中转录的LINE RNA逆转录成DNA，然后整合到宿主染色体中。虽然LINE编码的蛋白质在逆转录转座中的作用已经被揭示，但宿主编码的蛋白质的参与还没有得到很好的研究。在这里，我们提出的遗传证据表明，在修复DNA双链断裂的主机编码的蛋白质参与LINE反转录转座。

Long interspersed elements (LINEs) are transposable elements that mobilize and amplify their own copies within eukaryotic genomes. LINEs mobilize via a mechanism called retrotransposition, in which transcribed LINE RNA is reverse transcribed into DNA that is then integrated into the host chromosome. Although the role of LINE-encoded proteins in retrotransposition has been revealed, the participation of host-encoded proteins has not been well investigated. Here, we present genetic evidence that the host-encoded proteins involved in repair of DNA double-strand breaks participate in LINE retrotransposition.