Eiucidation of the mobilization mechanism of transposab leel ements

长穿插元件（LINEs）是在真核生物基因组中调动和扩增自身拷贝的转座元件。细胞系通过一种称为逆转录转位的机制进行动员，在这种机制中，转录的细胞系RNA被逆转录成DNA，然后整合到宿主染色体中。虽然行编码蛋白在逆转录中的作用已被揭示，但宿主编码蛋白的参与尚未得到很好的研究。在这里，我们提出遗传证据，宿主编码的蛋白质参与修复DNA双链断裂参与LINE反转录转位。

Long interspersed elements (LINEs) are transposable elements that mobilize and amplify their own copies within eukaryotic genomes. LINEs mobilize via a mechanism called retrotransposition, in which transcribed LINE RNA is reverse transcribed into DNA that is then integrated into the host chromosome. Although the role of LINE-encoded proteins in retrotransposition has been revealed, the participation of host-encoded proteins has not been well investigated. Here, we present genetic evidence that the host-encoded proteins involved in repair of DNA double-strand breaks participate in LINE retrotransposition.