Eiucidation of the mobilization mechanism of transposab leel ements

转座子元件动员机制的阐明

• 批准号: 21770002
• 负责人: KAJIKAWA Masaki
• 金额: $ 2.91万
• 依托单位: Tokyo Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21770002/
• 关键词: 転移因子; レトロトランスポゾン; 反復配列; レトロポゾン

Long interspersed elements (LINEs) are transposable elements that mobilize and amplify their own copies within eukaryotic genomes. LINEs mobilize via a mechanism called retrotransposition, in which transcribed LINE RNA is reverse transcribed into DNA that is then integrated into the host chromosome. Although the role of LINE-encoded proteins in retrotransposition has been revealed, the participation of host-encoded proteins has not been well investigated. Here, we present genetic evidence that the host-encoded proteins involved in repair of DNA double-strand breaks participate in LINE retrotransposition.

长散布元件（LINES）是在真核生物基因组中移动和扩增其自身拷贝的转座元件。LINE通过一种称为逆转录转座的机制移动，其中转录的LINE RNA逆转录成DNA，然后整合到宿主染色体中。尽管LINE编码的蛋白质在逆转录转座中的作用已被揭示，但宿主编码的蛋白质的参与尚未得到很好的研究。在这里，我们提出的遗传证据表明，在修复DNA双链断裂的主机编码的蛋白质参与LINE反转录转座。

项目成果

Host factors are involved in the 5' joining of the LINE integration.

主机因素参与 LINE 集成的 5 连接。

• 发表时间: 2010
• 作者: Kajikawa M.Yamaguchi K.;Okada N.
• 通讯作者: Okada N.

Genetic evidence that the non-homologous end-joining repair pathway is involved in LINE retrotransposition.

• DOI: 10.1371/journal.pgen.1000461
• 发表时间: 2009-04
• 期刊: PLoS genetics
• 影响因子: 4.5
• 作者: Suzuki J;Yamaguchi K;Kajikawa M;Ichiyanagi K;Adachi N;Koyama H;Takeda S;Okada N
• 通讯作者: Okada N

Host factors are involved in the 5' joining of the LINE integration

LINE 集成的 5 连接涉及主机因素

• 发表时间: 2010
• 作者: Masaki Kajikawa;Katsumi Yamaguchi;Norihiro Okada
• 通讯作者: Norihiro Okada